Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae
Abstract Background Swine dysentery (SD) is a severe infectious disease with a relevant impact on pig production usually caused by Brachyspira hyodysenteriae, although B. hampsonii causes an identical clinical picture. SD control relies on antimicrobials, good management practices and strict biosecu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-01-01
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| Series: | Porcine Health Management |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40813-021-00244-9 |
| _version_ | 1819229041481220096 |
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| author | Manuel Gómez-García Héctor Argüello Lucía Pérez-Pérez Clara Vega Héctor Puente Óscar Mencía-Ares Pedro Rubio Ana Carvajal |
| author_facet | Manuel Gómez-García Héctor Argüello Lucía Pérez-Pérez Clara Vega Héctor Puente Óscar Mencía-Ares Pedro Rubio Ana Carvajal |
| author_sort | Manuel Gómez-García |
| collection | DOAJ |
| description | Abstract Background Swine dysentery (SD) is a severe infectious disease with a relevant impact on pig production usually caused by Brachyspira hyodysenteriae, although B. hampsonii causes an identical clinical picture. SD control relies on antimicrobials, good management practices and strict biosecurity with cleaning and disinfection as crucial tools to avoid the pathogen transmission. This study evaluates the in-vitro efficacy of an array of commercial disinfectants against a collection of B. hyodysenteriae isolates using broth tests. The efficacy of cleaning and disinfection protocols was also evaluated on two farms with endemic SD using surface swabs collected in emptied pens before and after cleaning and disinfection procedures, using both real-time PCR and bacterial microbiological culture. Results Most of the commercial disinfectants evaluated were effective against all B. hyodysenteriae isolates tested, with a reduction of more than 5.00 log10 CFU/mL (bactericidal efficacy of 99.999%). However, some isolates exhibited reduced susceptibility to Virkon-S and Limoseptic disinfectants. The evaluation of cleaning and disinfection protocols on farms with SD outbreaks showed that approximately half the pens tested (n = 25) were positive by real-time PCR after pigs removal (mean B. hyodysenteriae counts 5.72 ± 1.04 log10 CFU/mL) while almost 20% of the pens remained positive after cleaning (n = 7) and disinfection (n = 5) procedures although with significantly lower, mean estimates (4.31 ± 0.43 log10 CFU/mL and 4.01 ± 0.55 log10 CFU/mL, respectively). Conclusions These results show the efficacy of disinfectants against B. hyodysenteriae but also stress the need to implement adequately the cleaning and disinfection protocols on pig farms and review and revise their efficiency periodically. |
| first_indexed | 2024-12-23T11:06:52Z |
| format | Article |
| id | doaj.art-317b30ae265946278a2dabed7c653479 |
| institution | Directory Open Access Journal |
| issn | 2055-5660 |
| language | English |
| last_indexed | 2024-12-23T11:06:52Z |
| publishDate | 2022-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Porcine Health Management |
| spelling | doaj.art-317b30ae265946278a2dabed7c6534792022-12-21T17:49:27ZengBMCPorcine Health Management2055-56602022-01-01811810.1186/s40813-021-00244-9Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriaeManuel Gómez-García0Héctor Argüello1Lucía Pérez-Pérez2Clara Vega3Héctor Puente4Óscar Mencía-Ares5Pedro Rubio6Ana Carvajal7Department of Animal Health, Faculty of Veterinary Medicine, Universidad de LeónDepartment of Animal Health, Faculty of Veterinary Medicine, Universidad de LeónDepartment of Animal Health, Faculty of Veterinary Medicine, Universidad de LeónDepartment of Animal Health, Faculty of Veterinary Medicine, Universidad de LeónDepartment of Animal Health, Faculty of Veterinary Medicine, Universidad de LeónDepartment of Animal Health, Faculty of Veterinary Medicine, Universidad de LeónDepartment of Animal Health, Faculty of Veterinary Medicine, Universidad de LeónDepartment of Animal Health, Faculty of Veterinary Medicine, Universidad de LeónAbstract Background Swine dysentery (SD) is a severe infectious disease with a relevant impact on pig production usually caused by Brachyspira hyodysenteriae, although B. hampsonii causes an identical clinical picture. SD control relies on antimicrobials, good management practices and strict biosecurity with cleaning and disinfection as crucial tools to avoid the pathogen transmission. This study evaluates the in-vitro efficacy of an array of commercial disinfectants against a collection of B. hyodysenteriae isolates using broth tests. The efficacy of cleaning and disinfection protocols was also evaluated on two farms with endemic SD using surface swabs collected in emptied pens before and after cleaning and disinfection procedures, using both real-time PCR and bacterial microbiological culture. Results Most of the commercial disinfectants evaluated were effective against all B. hyodysenteriae isolates tested, with a reduction of more than 5.00 log10 CFU/mL (bactericidal efficacy of 99.999%). However, some isolates exhibited reduced susceptibility to Virkon-S and Limoseptic disinfectants. The evaluation of cleaning and disinfection protocols on farms with SD outbreaks showed that approximately half the pens tested (n = 25) were positive by real-time PCR after pigs removal (mean B. hyodysenteriae counts 5.72 ± 1.04 log10 CFU/mL) while almost 20% of the pens remained positive after cleaning (n = 7) and disinfection (n = 5) procedures although with significantly lower, mean estimates (4.31 ± 0.43 log10 CFU/mL and 4.01 ± 0.55 log10 CFU/mL, respectively). Conclusions These results show the efficacy of disinfectants against B. hyodysenteriae but also stress the need to implement adequately the cleaning and disinfection protocols on pig farms and review and revise their efficiency periodically.https://doi.org/10.1186/s40813-021-00244-9Swine dysenteryDisinfectionCleaningInternal biosecurity |
| spellingShingle | Manuel Gómez-García Héctor Argüello Lucía Pérez-Pérez Clara Vega Héctor Puente Óscar Mencía-Ares Pedro Rubio Ana Carvajal Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae Porcine Health Management Swine dysentery Disinfection Cleaning Internal biosecurity |
| title | Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae |
| title_full | Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae |
| title_fullStr | Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae |
| title_full_unstemmed | Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae |
| title_short | Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae |
| title_sort | combined in vitro and on farm evaluation of commercial disinfectants used against brachyspira hyodysenteriae |
| topic | Swine dysentery Disinfection Cleaning Internal biosecurity |
| url | https://doi.org/10.1186/s40813-021-00244-9 |
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