Surface cysteine to serine substitutions in IL-18 reduce aggregation and enhance activity

Surface cysteine to serine substitutions in IL-18 reduce aggregation and enhance activity

Background Interleukin-18 (IL-18) is prone to form multimers resulting in inactive aggregates, making this cytokine unstable for clinical use. Therefore, mutations have been introduced into recombinant IL-18 to overcome this issue. Methods To prevent the formation of disulfide bonds between the IL-1...

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Bibliographic Details
Main Authors: Jirakrit Saetang, Niran Roongsawang, Surasak Sangkhathat, Supayang Piyawan Voravuthikunchai, Natnaree Sangkaew, Napat Prompat, Teerapol Srichana, Varomyalin Tipmanee
Format: Article
Language:English
Published: PeerJ Inc. 2022-07-01
Series:PeerJ
Subjects:
Interleukin-18
Surface cysteine
Aggregation
Interferon-γ
Molecular dynamic simulation
Online Access:https://peerj.com/articles/13626.pdf

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https://peerj.com/articles/13626.pdf

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