Expression and Surface Display of an Acidic Cold-Active Chitosanase in <i>Pichia pastoris</i> Using Multi-Copy Expression and High-Density Cultivation

Expression and Surface Display of an Acidic Cold-Active Chitosanase in <i>Pichia pastoris</i> Using Multi-Copy Expression and High-Density Cultivation

Chitosanase hydrolyzes β-(1,4)-linked glycosidic bonds are used in chitosan chains to release oligosaccharide mixtures. Here, we cloned and expressed a cold-adapted chitosanase (CDA, Genbank: MW094131) using multi-copy expression plasmids (CDA1/2/3/4) in <i>Pichia pastoris</i>. We identi...

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Bibliographic Details
Main Authors: Yanhong Peng, Yaping Wang, Xiaoyan Liu, Ronghua Zhou, Xianqing Liao, Yong Min, Lixin Ma, Ying Wang, Ben Rao
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Molecules
Subjects:
chitosanase
chiton oligosaccharides
high-density fermentation
multi-copy strains
<i>Pichia pastoris</i>
displayed enzyme
Online Access:https://www.mdpi.com/1420-3049/27/3/800

Internet

https://www.mdpi.com/1420-3049/27/3/800

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