WDR43 is a potential diagnostic biomarker and therapeutic target for osteoarthritis complicated with Parkinson’s disease

Osteoarthritis (OA) and Parkinson’s disease (PD) are on the rise and greatly impact the quality of individuals’ lives. Although accumulating evidence indicates a relationship between OA and PD, the particular interactions connecting the two diseases have not been thoroughly examined. Therefore, this...

Full description

Bibliographic Details
Main Authors: Hongquan Heng, Jie Liu, Mingwei Hu, Dazhuang Li, Wenxing Su, Jian Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-11-01
Series:Frontiers in Cellular Neuroscience
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fncel.2022.1013745/full
_version_ 1797984533208891392
author Hongquan Heng
Hongquan Heng
Jie Liu
Jie Liu
Mingwei Hu
Dazhuang Li
Wenxing Su
Jian Li
author_facet Hongquan Heng
Hongquan Heng
Jie Liu
Jie Liu
Mingwei Hu
Dazhuang Li
Wenxing Su
Jian Li
author_sort Hongquan Heng
collection DOAJ
description Osteoarthritis (OA) and Parkinson’s disease (PD) are on the rise and greatly impact the quality of individuals’ lives. Although accumulating evidence indicates a relationship between OA and PD, the particular interactions connecting the two diseases have not been thoroughly examined. Therefore, this study explored the association through genetic characterization and functional enrichment. Four datasets (GSE55235, GSE12021, GSE7621, and GSE42966) were chosen for assessment and validation from the Gene Expression Omnibus (GEO) database. Weighted Gene Co-Expression Network Analysis (WGCNA) was implemented to determine the most relevant genes for clinical features. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were carried out to explore the biological processes of common genes, and to display the interrelationships between common genes, the STRING database and the application Molecular Complex Detection Algorithm (MCODE) of Cytoscape software were leveraged to get hub genes. By intersecting the common genes with the differentially expressed genes (DEGs) acquired from GSE12021 and GSE42966, the hub genes were identified. Finally, we validated the diagnostic efficacy of hub genes and explored their correlation with 22 immune infiltrating cells. As a consequence, we discovered 71 common genes, most of which were functionally enriched in antigen processing and presentation, mitochondrial translation, the mRNA surveillance pathway, and nucleocytoplasmic transport. Furthermore, WDR43 was found by intersecting eight hub genes with 28 DEGs from the two validation datasets. Receiver Operating Characteristic (ROC) implied the diagnostic role of WDR43 in OA and PD. Immune infiltration research revealed that T-cell regulatory (Tregs), monocytes, and mast cells resting were associated with the pathogenesis of OA and PD. WDR43 may provide key insights into the relationship between OA and PD.
first_indexed 2024-04-11T07:04:16Z
format Article
id doaj.art-31c37f4640fb44448f8d63db78ffcdf3
institution Directory Open Access Journal
issn 1662-5102
language English
last_indexed 2024-04-11T07:04:16Z
publishDate 2022-11-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Cellular Neuroscience
spelling doaj.art-31c37f4640fb44448f8d63db78ffcdf32022-12-22T04:38:28ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022022-11-011610.3389/fncel.2022.10137451013745WDR43 is a potential diagnostic biomarker and therapeutic target for osteoarthritis complicated with Parkinson’s diseaseHongquan Heng0Hongquan Heng1Jie Liu2Jie Liu3Mingwei Hu4Dazhuang Li5Wenxing Su6Jian Li7Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Plastic and Burn Surgery, The Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, ChinaDepartment of Orthopedics, Liyang People’s Hospital, Liyang, ChinaDepartment of Orthopedics, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, ChinaDepartment of Neurology, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Orthopedics, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, ChinaDepartment of Plastic and Burn Surgery, The Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaOsteoarthritis (OA) and Parkinson’s disease (PD) are on the rise and greatly impact the quality of individuals’ lives. Although accumulating evidence indicates a relationship between OA and PD, the particular interactions connecting the two diseases have not been thoroughly examined. Therefore, this study explored the association through genetic characterization and functional enrichment. Four datasets (GSE55235, GSE12021, GSE7621, and GSE42966) were chosen for assessment and validation from the Gene Expression Omnibus (GEO) database. Weighted Gene Co-Expression Network Analysis (WGCNA) was implemented to determine the most relevant genes for clinical features. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were carried out to explore the biological processes of common genes, and to display the interrelationships between common genes, the STRING database and the application Molecular Complex Detection Algorithm (MCODE) of Cytoscape software were leveraged to get hub genes. By intersecting the common genes with the differentially expressed genes (DEGs) acquired from GSE12021 and GSE42966, the hub genes were identified. Finally, we validated the diagnostic efficacy of hub genes and explored their correlation with 22 immune infiltrating cells. As a consequence, we discovered 71 common genes, most of which were functionally enriched in antigen processing and presentation, mitochondrial translation, the mRNA surveillance pathway, and nucleocytoplasmic transport. Furthermore, WDR43 was found by intersecting eight hub genes with 28 DEGs from the two validation datasets. Receiver Operating Characteristic (ROC) implied the diagnostic role of WDR43 in OA and PD. Immune infiltration research revealed that T-cell regulatory (Tregs), monocytes, and mast cells resting were associated with the pathogenesis of OA and PD. WDR43 may provide key insights into the relationship between OA and PD.https://www.frontiersin.org/articles/10.3389/fncel.2022.1013745/fullosteoarthritisParkinson’s diseaseweighted gene co-expression network analysisimmune cell infiltrationhub gene
spellingShingle Hongquan Heng
Hongquan Heng
Jie Liu
Jie Liu
Mingwei Hu
Dazhuang Li
Wenxing Su
Jian Li
WDR43 is a potential diagnostic biomarker and therapeutic target for osteoarthritis complicated with Parkinson’s disease
Frontiers in Cellular Neuroscience
osteoarthritis
Parkinson’s disease
weighted gene co-expression network analysis
immune cell infiltration
hub gene
title WDR43 is a potential diagnostic biomarker and therapeutic target for osteoarthritis complicated with Parkinson’s disease
title_full WDR43 is a potential diagnostic biomarker and therapeutic target for osteoarthritis complicated with Parkinson’s disease
title_fullStr WDR43 is a potential diagnostic biomarker and therapeutic target for osteoarthritis complicated with Parkinson’s disease
title_full_unstemmed WDR43 is a potential diagnostic biomarker and therapeutic target for osteoarthritis complicated with Parkinson’s disease
title_short WDR43 is a potential diagnostic biomarker and therapeutic target for osteoarthritis complicated with Parkinson’s disease
title_sort wdr43 is a potential diagnostic biomarker and therapeutic target for osteoarthritis complicated with parkinson s disease
topic osteoarthritis
Parkinson’s disease
weighted gene co-expression network analysis
immune cell infiltration
hub gene
url https://www.frontiersin.org/articles/10.3389/fncel.2022.1013745/full
work_keys_str_mv AT hongquanheng wdr43isapotentialdiagnosticbiomarkerandtherapeutictargetforosteoarthritiscomplicatedwithparkinsonsdisease
AT hongquanheng wdr43isapotentialdiagnosticbiomarkerandtherapeutictargetforosteoarthritiscomplicatedwithparkinsonsdisease
AT jieliu wdr43isapotentialdiagnosticbiomarkerandtherapeutictargetforosteoarthritiscomplicatedwithparkinsonsdisease
AT jieliu wdr43isapotentialdiagnosticbiomarkerandtherapeutictargetforosteoarthritiscomplicatedwithparkinsonsdisease
AT mingweihu wdr43isapotentialdiagnosticbiomarkerandtherapeutictargetforosteoarthritiscomplicatedwithparkinsonsdisease
AT dazhuangli wdr43isapotentialdiagnosticbiomarkerandtherapeutictargetforosteoarthritiscomplicatedwithparkinsonsdisease
AT wenxingsu wdr43isapotentialdiagnosticbiomarkerandtherapeutictargetforosteoarthritiscomplicatedwithparkinsonsdisease
AT jianli wdr43isapotentialdiagnosticbiomarkerandtherapeutictargetforosteoarthritiscomplicatedwithparkinsonsdisease