CRISPR-Cas9-induced gene knockout in zebrafish

CRISPR-Cas9-induced gene knockout in zebrafish

Summary: The application of CRISPR has greatly facilitated genotype-phenotype studies of human disease models. In this protocol, we describe CRISPR-Cas9-induced gene knockout in zebrafish, utilizing purified Cas9 protein and in vitro-transcribed sgRNA. This protocol targets the PHLPP1 gene in an Ind...

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Bibliographic Details
Main Authors: Raghavender Medishetti, Keerthana Balamurugan, Krishnaveni Yadavalli, Rita Rani, Aarti Sevilimedu, Anil Kumar Challa, Kishore Parsa, Kiranam Chatti
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Genetics
Sequencing
Model organisms
Molecular biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006591

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http://www.sciencedirect.com/science/article/pii/S2666166722006591

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