Protocol for preparing Thiopropyl Sepharose resin used for capturing S-nitrosylated proteins

Protocol for preparing Thiopropyl Sepharose resin used for capturing S-nitrosylated proteins

Summary: S-nitrosothiol (SNO)-Resin Assisted Capture (SNO-RAC) relies on a Thiopropyl Sepharose resin to identify S-nitrosylated proteins (SNO-proteins) and sites of S-nitrosylation. Here, we present a protocol for preparing Thiopropyl Sepharose resin with efficiency of SNO-protein capture comparabl...

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Bibliographic Details
Main Authors: Puneet Seth, Alfred Hausladen, Richard T. Premont, Jonathan S. Stamler
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Signal Transduction
Protein Biochemistry
Chemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003970

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723003970

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