Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.

A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath). Thus, we examined several isothermal amplification methods focusing on simplicity, cost, sen...

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Bibliographic Details
Main Authors: Yiping Zou, Michael Glenn Mason, Jose Ramon Botella
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0235216