Gene-targeted embryonic stem cells: real-time PCR assay for estimation of the number of neomycin selection cassettes
<p>Abstract</p> <p>In the preparation of transgenic murine ES cells it is important to verify the construct has a single insertion, because an ectopic neomycin phosphortransferase positive selection cassette (<it>NEO</it>) may cause a position effect. During a recent wo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-10-01
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| Series: | Biological Procedures Online |
| Online Access: | http://www.biologicalproceduresonline.com/content/13/1/10 |