Optimization of Ultrasound-Assisted Extraction of Ilex latifolia Thunb. Polysaccharide and Its Effect on the Inhibitory Activity of Xanthine Oxidase
Objective: To optimize the extraction process of Ilex latifolia Thunb. polysaccharide and analyze its inhibitory activity of xanthine oxidase. Methods: A response surface optimization ultrasound-assisted method was used to study the effects of material/liquid ratio, ultrasonic time, ultrasonic tempe...
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The editorial department of Science and Technology of Food Industry
2023-04-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070019 |
| _version_ | 1797838893973766144 |
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| author | Zhongying LU Mengqin ZHANG Xiang CHEN |
| author_facet | Zhongying LU Mengqin ZHANG Xiang CHEN |
| author_sort | Zhongying LU |
| collection | DOAJ |
| description | Objective: To optimize the extraction process of Ilex latifolia Thunb. polysaccharide and analyze its inhibitory activity of xanthine oxidase. Methods: A response surface optimization ultrasound-assisted method was used to study the effects of material/liquid ratio, ultrasonic time, ultrasonic temperature and ultrasound power on the yield of Ilex latifolia Thunb. polysaccharide, and the inhibition rate of Ilex latifolia Thunb. polysaccharide on xanthine oxidase and its kinetic constant Ki were determined by in vitro experiments. Results: The optimized conditions of the response surface test were: The material/liquid ratio was 1:25 g/mL, the ultrasonic time was 60 min, the ultrasonic temperature was 40 ℃, the ultrasonic power was 260 W and the yield rate was 5.85%. The inhibition rate of xanthine oxidase by Ilex latifolia Thunb. polysaccharide was concentration-dependent with an IC50 of 1.28 mg/mL, the type of inhibition of xanthine oxidase was competitive and reversible with an inhibition kinetic constant Ki of 0.62 mg/mL. Conclusion: Ilex latifolia Thunb. polysaccharide could inhibit the activity of xanthine oxidase and its could be studied as a potential food or health product for the prevention of hyperuricemia, with a view to providing theoretical data on the comprehensive utilization of Ilex latifolia Thunb.. |
| first_indexed | 2024-04-09T15:49:23Z |
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| id | doaj.art-32f3947e259349e18a42a980adf706ee |
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| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-09T15:49:23Z |
| publishDate | 2023-04-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-32f3947e259349e18a42a980adf706ee2023-04-26T12:48:50ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-04-0144822823510.13386/j.issn1002-0306.20220700192022070019-8Optimization of Ultrasound-Assisted Extraction of Ilex latifolia Thunb. Polysaccharide and Its Effect on the Inhibitory Activity of Xanthine OxidaseZhongying LU0Mengqin ZHANG1Xiang CHEN2Department of Food Engineering, Guizhou Vocational College of Foodstuff Engineering, Guiyang 551400, ChinaInstitute of Material and Chemical Engineering, Tongren University, Tongren 554300, ChinaDepartment of Food Engineering, Guizhou Vocational College of Foodstuff Engineering, Guiyang 551400, ChinaObjective: To optimize the extraction process of Ilex latifolia Thunb. polysaccharide and analyze its inhibitory activity of xanthine oxidase. Methods: A response surface optimization ultrasound-assisted method was used to study the effects of material/liquid ratio, ultrasonic time, ultrasonic temperature and ultrasound power on the yield of Ilex latifolia Thunb. polysaccharide, and the inhibition rate of Ilex latifolia Thunb. polysaccharide on xanthine oxidase and its kinetic constant Ki were determined by in vitro experiments. Results: The optimized conditions of the response surface test were: The material/liquid ratio was 1:25 g/mL, the ultrasonic time was 60 min, the ultrasonic temperature was 40 ℃, the ultrasonic power was 260 W and the yield rate was 5.85%. The inhibition rate of xanthine oxidase by Ilex latifolia Thunb. polysaccharide was concentration-dependent with an IC50 of 1.28 mg/mL, the type of inhibition of xanthine oxidase was competitive and reversible with an inhibition kinetic constant Ki of 0.62 mg/mL. Conclusion: Ilex latifolia Thunb. polysaccharide could inhibit the activity of xanthine oxidase and its could be studied as a potential food or health product for the prevention of hyperuricemia, with a view to providing theoretical data on the comprehensive utilization of Ilex latifolia Thunb..http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070019ilex latifolia thunb. (ilt)polysaccharoseresponse surface methodology (rsm)xanthine oxidase (xod)inhibition |
| spellingShingle | Zhongying LU Mengqin ZHANG Xiang CHEN Optimization of Ultrasound-Assisted Extraction of Ilex latifolia Thunb. Polysaccharide and Its Effect on the Inhibitory Activity of Xanthine Oxidase Shipin gongye ke-ji ilex latifolia thunb. (ilt) polysaccharose response surface methodology (rsm) xanthine oxidase (xod) inhibition |
| title | Optimization of Ultrasound-Assisted Extraction of Ilex latifolia Thunb. Polysaccharide and Its Effect on the Inhibitory Activity of Xanthine Oxidase |
| title_full | Optimization of Ultrasound-Assisted Extraction of Ilex latifolia Thunb. Polysaccharide and Its Effect on the Inhibitory Activity of Xanthine Oxidase |
| title_fullStr | Optimization of Ultrasound-Assisted Extraction of Ilex latifolia Thunb. Polysaccharide and Its Effect on the Inhibitory Activity of Xanthine Oxidase |
| title_full_unstemmed | Optimization of Ultrasound-Assisted Extraction of Ilex latifolia Thunb. Polysaccharide and Its Effect on the Inhibitory Activity of Xanthine Oxidase |
| title_short | Optimization of Ultrasound-Assisted Extraction of Ilex latifolia Thunb. Polysaccharide and Its Effect on the Inhibitory Activity of Xanthine Oxidase |
| title_sort | optimization of ultrasound assisted extraction of ilex latifolia thunb polysaccharide and its effect on the inhibitory activity of xanthine oxidase |
| topic | ilex latifolia thunb. (ilt) polysaccharose response surface methodology (rsm) xanthine oxidase (xod) inhibition |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070019 |
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