Rapid Detection and Quantification of Rhynchosporium secalis in Barley Using a Polymerase Chain Reaction
PCR primers for diagnosis of Rhynchosporium secalis in seed samples of barley were developed. For the quantification of the pathogen in seed samples a real-time PCR with SYBR Green approach was used. Amounts from 1.8 to 419.1 pg of R. secalis DNA per 100 ng of total DNA were detected in 18 samples o...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2006-09-01
|
| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-200603-0005_rapid-detection-and-quantification-of-rhynchosporium-secalis-in-barley-using-a-polymerase-chain-reaction.php |