Quantification of Densities of Bacterial Endosymbionts of Insects by Real-time PCR
Increased attention has been paid to the endosymbiotic bacteria of insects. Because most insect endosymbionts are uncultivable, quantitative PCR (qPCR) is a practical and convenient method to quantify endosymbiont titers. Here we report a protocol for real-time qPCR based on SYBR Green I fluorescenc...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2017-10-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=2566&type=0 |