Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysis

Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysis

Summary: Patient-derived organoids (PDOs) are ideal ex vivo model systems to study cancer progression and drug resistance mechanisms. Here, we present a protocol for measuring drug efficacy in three-dimensional (3D) high-grade serous ovarian cancer PDO cultures through quantification of cytotoxicity...

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Bibliographic Details
Main Authors: Aikaterini Skorda, Anna Røssberg Lauridsen, Kaisa Huhtinen, Alexandra Lahtinen, Wojciech Senkowski, Jaana Oikkonen, Johanna Hynninen, Sampsa Hautaniemi, Tuula Kallunki
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cancer
High-Throughput Screening
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723006500

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http://www.sciencedirect.com/science/article/pii/S2666166723006500

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