Characterization of Genomic, Physiological, and Probiotic Features of <i>Lactiplantibacillus plantarum</i> JS21 Strain Isolated from Traditional Fermented Jiangshui
This study aimed to understand the genetic and metabolic traits of a <i>Lactiplantibacillus plantarum</i> JS21 strain and its probiotic abilities through laboratory tests and computer analysis. <i>L. plantarum</i> JS21 was isolated from a traditional fermented food known as “...
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MDPI AG
2024-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/7/1082 |
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| author | Yang Liu Shanshan Wang Ling Wang Hongzhao Lu Tao Zhang Wenxian Zeng |
| author_facet | Yang Liu Shanshan Wang Ling Wang Hongzhao Lu Tao Zhang Wenxian Zeng |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | This study aimed to understand the genetic and metabolic traits of a <i>Lactiplantibacillus plantarum</i> JS21 strain and its probiotic abilities through laboratory tests and computer analysis. <i>L. plantarum</i> JS21 was isolated from a traditional fermented food known as “Jiangshui” in Hanzhong city. In this research, the complete genetic makeup of JS21 was determined using Illumina and PacBio technologies. The JS21 genome consisted of a 3.423 Mb circular chromosome and five plasmids. It was found to contain 3023 protein-coding genes, 16 tRNA genes, 64 rRNA operons, 40 non-coding RNA genes, 264 pseudogenes, and six CRISPR array regions. The GC content of the genome was 44.53%. Additionally, the genome harbored three complete prophages. The evolutionary relationship and the genome collinearity of JS21 were compared with other <i>L. plantarum</i> strains. The resistance genes identified in JS21 were inherent. Enzyme genes involved in the Embden–Meyerhof–Parnas (EMP) and phosphoketolase (PK) pathways were detected, indicating potential for facultative heterofermentative pathways. JS21 possessed bacteriocins <i>plnE</i>/<i>plnF</i> genes and genes for polyketide and terpenoid assembly, possibly contributing to its antibacterial properties against <i>Escherichia coli</i> (ATCC 25922), <i>Escherichia coli</i> (K88), <i>Staphylococcus aureus</i> (CMCC 26003), and <i>Listeria monocytogenes</i> (CICC 21635). Furthermore, JS21 carried genes for Na<sup>+</sup>/H<sup>+</sup> antiporters, F<sub>0</sub>F<sub>1</sub> ATPase, and other stress resistance genes, which may account for its ability to withstand simulated conditions of the human gastrointestinal tract <i>in vitro</i>. The high hydrophobicity of its cell surface suggested the potential for intestinal colonization. Overall, <i>L. plantarum</i> JS21 exhibited probiotic traits as evidenced by laboratory experiments and computational analysis, suggesting its suitability as a dietary supplement. |
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| language | English |
| last_indexed | 2024-04-24T10:44:15Z |
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| spelling | doaj.art-3317ab6458a741d09dfbe427aa3aa65e2024-04-12T13:18:37ZengMDPI AGFoods2304-81582024-04-01137108210.3390/foods13071082Characterization of Genomic, Physiological, and Probiotic Features of <i>Lactiplantibacillus plantarum</i> JS21 Strain Isolated from Traditional Fermented JiangshuiYang Liu0Shanshan Wang1Ling Wang2Hongzhao Lu3Tao Zhang4Wenxian Zeng5School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, ChinaThis study aimed to understand the genetic and metabolic traits of a <i>Lactiplantibacillus plantarum</i> JS21 strain and its probiotic abilities through laboratory tests and computer analysis. <i>L. plantarum</i> JS21 was isolated from a traditional fermented food known as “Jiangshui” in Hanzhong city. In this research, the complete genetic makeup of JS21 was determined using Illumina and PacBio technologies. The JS21 genome consisted of a 3.423 Mb circular chromosome and five plasmids. It was found to contain 3023 protein-coding genes, 16 tRNA genes, 64 rRNA operons, 40 non-coding RNA genes, 264 pseudogenes, and six CRISPR array regions. The GC content of the genome was 44.53%. Additionally, the genome harbored three complete prophages. The evolutionary relationship and the genome collinearity of JS21 were compared with other <i>L. plantarum</i> strains. The resistance genes identified in JS21 were inherent. Enzyme genes involved in the Embden–Meyerhof–Parnas (EMP) and phosphoketolase (PK) pathways were detected, indicating potential for facultative heterofermentative pathways. JS21 possessed bacteriocins <i>plnE</i>/<i>plnF</i> genes and genes for polyketide and terpenoid assembly, possibly contributing to its antibacterial properties against <i>Escherichia coli</i> (ATCC 25922), <i>Escherichia coli</i> (K88), <i>Staphylococcus aureus</i> (CMCC 26003), and <i>Listeria monocytogenes</i> (CICC 21635). Furthermore, JS21 carried genes for Na<sup>+</sup>/H<sup>+</sup> antiporters, F<sub>0</sub>F<sub>1</sub> ATPase, and other stress resistance genes, which may account for its ability to withstand simulated conditions of the human gastrointestinal tract <i>in vitro</i>. The high hydrophobicity of its cell surface suggested the potential for intestinal colonization. Overall, <i>L. plantarum</i> JS21 exhibited probiotic traits as evidenced by laboratory experiments and computational analysis, suggesting its suitability as a dietary supplement.https://www.mdpi.com/2304-8158/13/7/1082Jiangshui<i>Lactiplantibacillus plantarum</i>genomeprobioticbacteriocin |
| spellingShingle | Yang Liu Shanshan Wang Ling Wang Hongzhao Lu Tao Zhang Wenxian Zeng Characterization of Genomic, Physiological, and Probiotic Features of <i>Lactiplantibacillus plantarum</i> JS21 Strain Isolated from Traditional Fermented Jiangshui Foods Jiangshui <i>Lactiplantibacillus plantarum</i> genome probiotic bacteriocin |
| title | Characterization of Genomic, Physiological, and Probiotic Features of <i>Lactiplantibacillus plantarum</i> JS21 Strain Isolated from Traditional Fermented Jiangshui |
| title_full | Characterization of Genomic, Physiological, and Probiotic Features of <i>Lactiplantibacillus plantarum</i> JS21 Strain Isolated from Traditional Fermented Jiangshui |
| title_fullStr | Characterization of Genomic, Physiological, and Probiotic Features of <i>Lactiplantibacillus plantarum</i> JS21 Strain Isolated from Traditional Fermented Jiangshui |
| title_full_unstemmed | Characterization of Genomic, Physiological, and Probiotic Features of <i>Lactiplantibacillus plantarum</i> JS21 Strain Isolated from Traditional Fermented Jiangshui |
| title_short | Characterization of Genomic, Physiological, and Probiotic Features of <i>Lactiplantibacillus plantarum</i> JS21 Strain Isolated from Traditional Fermented Jiangshui |
| title_sort | characterization of genomic physiological and probiotic features of i lactiplantibacillus plantarum i js21 strain isolated from traditional fermented jiangshui |
| topic | Jiangshui <i>Lactiplantibacillus plantarum</i> genome probiotic bacteriocin |
| url | https://www.mdpi.com/2304-8158/13/7/1082 |
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