Surfactant Supplementation to Enhance the Production of Vitamin K2 Metabolites in Shake Flask Cultures Using Escherichia sp. Mutant FM3-1709
The effects of the addition of various surfactants on the cell growth and production of vitamin K2 metabolites, such as intracellular menaquinone-4 (MK-4), extracellular MK-4, intracellular MK-6 and extracellular MK-6, were studied in the submerged culture of Escherichia sp. All of the added surfact...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2014-01-01
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| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/186420 |
| Summary: | The effects of the addition of various surfactants on the cell growth and production of vitamin K2 metabolites, such as intracellular menaquinone-4 (MK-4), extracellular MK-4, intracellular MK-6 and extracellular MK-6, were studied in the submerged culture of Escherichia sp. All of the added surfactants caused the extension of the exponential phase. Betaine, polyoxyethylene oleyl ether and Tween-80 were favourable to the cell growth of Escherichia sp. mutant strain FM3-1709. The highest cell growth (Xmax), biomass production rate (Qx) and biomass yield (Yx/s) were (12.6±0.2) g/L, (0.21±0.01) g/(L·h) and (2.42± 0.02) g/g, respectively. The results show that the addition of all surfactants led to a lower production of intracellular MK-4, whereas the production of extracellular MK-4 increased remarkably. Among the five investigated surfactants, the addition of the nonionic surfactant polyoxyethylene oleyl ether (1.0 g/L) led to the highest production of extracellular MK-4 ((33.6±0.4) mg/L), MK-6 ((2.56±0.07) mg/L) and the highest yield of total MK-4
((47.6±0.4) mg/L) and MK-6 ((6.0±0.1) mg/L). The addition of polyoxyethylene oleyl ether proved to be more beneficial for the secretion of MK-4 than MK-6. |
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| ISSN: | 1330-9862 1334-2606 |