Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler Chickens
The present and future high demand of common cereals as corn and wheat encourage the development of feed processing technology that allows for the dietary inclusion of other cereals of low nutritional value in poultry feeding. Barley grains contain anti-nutritional factors that limit their dietary i...
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MDPI AG
2023-09-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/10/594 |
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| author | Doaa Ibrahim Hassainen I. El-sayed Elsabbagh R. Mahmoud Ghada I. Abd El-Rahman Shefaa M. Bazeed Abdelwahab A. Abdelwarith Aya Elgamal Samah S. Khalil Elsayed M. Younis Asmaa T. Y. Kishawy Simon J. Davies Abdallah E. Metwally |
| author_facet | Doaa Ibrahim Hassainen I. El-sayed Elsabbagh R. Mahmoud Ghada I. Abd El-Rahman Shefaa M. Bazeed Abdelwahab A. Abdelwarith Aya Elgamal Samah S. Khalil Elsayed M. Younis Asmaa T. Y. Kishawy Simon J. Davies Abdallah E. Metwally |
| author_sort | Doaa Ibrahim |
| collection | DOAJ |
| description | The present and future high demand of common cereals as corn and wheat encourage the development of feed processing technology that allows for the dietary inclusion of other cereals of low nutritional value in poultry feeding. Barley grains contain anti-nutritional factors that limit their dietary inclusion in the poultry industry. The treatment of barley with solid-state fermentation and exogenous enzymes (FBEs) provides a good alternative to common cereals. In this study, barley grains were subjected to solid-state microbial fermentation using <i>Lactobacillus plantarum</i>, <i>Bacillus subtilis</i> and exogenous fibrolytic enzymes. This study aimed to assess the impact of FBEs on growth, feed utilization efficiency, immune modulation, antioxidant status and the expression of intestinal barrier and nutrient transporter-related genes. One-day-old broiler chicks (Ross 308, <i>n</i> = 400) comprised four representative groups with ten replicates (10 chicks/replicate) and were fed corn-soybean meal basal diets with inclusions of FBEs at 0, 5, 10 and 15% for 38 days. Solid-state fermentation of barley grains with fibrolytic enzymes increased protein content, lowered crude fiber and reduced sugars compared to non-fermented barley gains. In consequence, the group fed FBEs10% had the superior feed utilization efficiency and body weight gain (increased by 4.7%) with higher levels of nutrient metabolizability, pancreatic digestive enzyme activities and low digesta viscosity. Notably, the group fed FBEs10% showed an increased villi height and a decreased crypt depth with a remarkable hyperactivity of duodenal glands. In addition, higher inclusion levels of FBEs boosted serum immune-related parameters and intestinal and breast muscle antioxidants status. Intestinal nutrient transporters encoding genes (GLUT-1, CAAT-1, LAT1 and PepT-1) and intestinal barriers encoding genes (MUC-2, JAM-2, occludin, claudins-1 and β-defensin 1) were upregulated with higher dietary FBEs levels. In conclusion, feeding on FBEs10% positively enhanced broiler chickens’ performance, feed efficiency and antioxidant status, and boosted intestinal barrier nutrient transporters encoding genes. |
| first_indexed | 2024-03-10T20:49:41Z |
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| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T20:49:41Z |
| publishDate | 2023-09-01 |
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| series | Veterinary Sciences |
| spelling | doaj.art-335615c6fced4f67bf5506687564d8c82023-11-19T18:25:41ZengMDPI AGVeterinary Sciences2306-73812023-09-01101059410.3390/vetsci10100594Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler ChickensDoaa Ibrahim0Hassainen I. El-sayed1Elsabbagh R. Mahmoud2Ghada I. Abd El-Rahman3Shefaa M. Bazeed4Abdelwahab A. Abdelwarith5Aya Elgamal6Samah S. Khalil7Elsayed M. Younis8Asmaa T. Y. Kishawy9Simon J. Davies10Abdallah E. Metwally11Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Badr University in Cairo (BUC), Cairo P.O. Box 4942301, EgyptDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Animal Histology and Anatomy, Faculty of Veterinary Medicine, Badr University in Cairo (BUC), Cairo P.O. Box 4942301, EgyptDepartment of biochemistry, drug information center, Zagazig University Hospitals, Zagazig University, Zagazig P.O. Box 44511, EgyptDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptAquaculture Nutrition Research Unit ANRU, Carna Research Station, Ryan Institute, College of Science and Engineering, University of Galway, H91 V8Y1 Galway, IrelandDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptThe present and future high demand of common cereals as corn and wheat encourage the development of feed processing technology that allows for the dietary inclusion of other cereals of low nutritional value in poultry feeding. Barley grains contain anti-nutritional factors that limit their dietary inclusion in the poultry industry. The treatment of barley with solid-state fermentation and exogenous enzymes (FBEs) provides a good alternative to common cereals. In this study, barley grains were subjected to solid-state microbial fermentation using <i>Lactobacillus plantarum</i>, <i>Bacillus subtilis</i> and exogenous fibrolytic enzymes. This study aimed to assess the impact of FBEs on growth, feed utilization efficiency, immune modulation, antioxidant status and the expression of intestinal barrier and nutrient transporter-related genes. One-day-old broiler chicks (Ross 308, <i>n</i> = 400) comprised four representative groups with ten replicates (10 chicks/replicate) and were fed corn-soybean meal basal diets with inclusions of FBEs at 0, 5, 10 and 15% for 38 days. Solid-state fermentation of barley grains with fibrolytic enzymes increased protein content, lowered crude fiber and reduced sugars compared to non-fermented barley gains. In consequence, the group fed FBEs10% had the superior feed utilization efficiency and body weight gain (increased by 4.7%) with higher levels of nutrient metabolizability, pancreatic digestive enzyme activities and low digesta viscosity. Notably, the group fed FBEs10% showed an increased villi height and a decreased crypt depth with a remarkable hyperactivity of duodenal glands. In addition, higher inclusion levels of FBEs boosted serum immune-related parameters and intestinal and breast muscle antioxidants status. Intestinal nutrient transporters encoding genes (GLUT-1, CAAT-1, LAT1 and PepT-1) and intestinal barriers encoding genes (MUC-2, JAM-2, occludin, claudins-1 and β-defensin 1) were upregulated with higher dietary FBEs levels. In conclusion, feeding on FBEs10% positively enhanced broiler chickens’ performance, feed efficiency and antioxidant status, and boosted intestinal barrier nutrient transporters encoding genes.https://www.mdpi.com/2306-7381/10/10/594solid state fermentationbarley grainsfibrolytic enzymesbroiler chickensintestinal barriersnutrient transporters |
| spellingShingle | Doaa Ibrahim Hassainen I. El-sayed Elsabbagh R. Mahmoud Ghada I. Abd El-Rahman Shefaa M. Bazeed Abdelwahab A. Abdelwarith Aya Elgamal Samah S. Khalil Elsayed M. Younis Asmaa T. Y. Kishawy Simon J. Davies Abdallah E. Metwally Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler Chickens Veterinary Sciences solid state fermentation barley grains fibrolytic enzymes broiler chickens intestinal barriers nutrient transporters |
| title | Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler Chickens |
| title_full | Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler Chickens |
| title_fullStr | Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler Chickens |
| title_full_unstemmed | Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler Chickens |
| title_short | Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler Chickens |
| title_sort | impacts of solid state fermented barley with fibrolytic exogenous enzymes on feed utilization and antioxidant status of broiler chickens |
| topic | solid state fermentation barley grains fibrolytic enzymes broiler chickens intestinal barriers nutrient transporters |
| url | https://www.mdpi.com/2306-7381/10/10/594 |
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