Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody
Using an immunoassay in combination with surface plasmon fluorescence spectroscopy (SPFS), we report the rapid detection of troponin I, a valuable biomarker for diagnosis of myocardial infarction. We discuss the implementation of (i) direct, (ii) sandwich, and (iii) competitive assay formats, based...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2020-10-01
|
| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.200871 |
| _version_ | 1818329808199745536 |
|---|---|
| author | Anıl Bozdogan Reham F. El-Kased Vanessa Jungbluth Wolfgang Knoll Jakub Dostalek Amal Kasry |
| author_facet | Anıl Bozdogan Reham F. El-Kased Vanessa Jungbluth Wolfgang Knoll Jakub Dostalek Amal Kasry |
| author_sort | Anıl Bozdogan |
| collection | DOAJ |
| description | Using an immunoassay in combination with surface plasmon fluorescence spectroscopy (SPFS), we report the rapid detection of troponin I, a valuable biomarker for diagnosis of myocardial infarction. We discuss the implementation of (i) direct, (ii) sandwich, and (iii) competitive assay formats, based on surface plasmon resonance and SPFS. To elucidate the results, we relate the experiments to orientation-dependent interaction of troponin I epitopes with respective immunoglobulin G antibodies. A limit of detection (LoD) of 19 pM, with 45 min readout time, was achieved using single monoclonal antibody that is specific for one epitope. The borderline between normal people and patients is 20 pM to 83 pM cTnI concentration, and upon the outbreak of acute myocardial infraction it can raise to 2 nM and levels at 20 nM for 6–8 days, therefore the achieved LoD covers most of the clinically relevant range. In addition, this system allows for the detection of troponin I using a single specific monoclonal antibody, which is highly beneficial in case of detection in real samples, where the protein has a complex form leading to hidden epitopes, thus paving the way towards a system that can improve early-stage screening of heart attacks. |
| first_indexed | 2024-12-13T12:53:56Z |
| format | Article |
| id | doaj.art-33ac9eebcb18486898e750ca69686f42 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-13T12:53:56Z |
| publishDate | 2020-10-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-33ac9eebcb18486898e750ca69686f422022-12-21T23:45:15ZengThe Royal SocietyRoyal Society Open Science2054-57032020-10-0171010.1098/rsos.200871200871Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibodyAnıl BozdoganReham F. El-KasedVanessa JungbluthWolfgang KnollJakub DostalekAmal KasryUsing an immunoassay in combination with surface plasmon fluorescence spectroscopy (SPFS), we report the rapid detection of troponin I, a valuable biomarker for diagnosis of myocardial infarction. We discuss the implementation of (i) direct, (ii) sandwich, and (iii) competitive assay formats, based on surface plasmon resonance and SPFS. To elucidate the results, we relate the experiments to orientation-dependent interaction of troponin I epitopes with respective immunoglobulin G antibodies. A limit of detection (LoD) of 19 pM, with 45 min readout time, was achieved using single monoclonal antibody that is specific for one epitope. The borderline between normal people and patients is 20 pM to 83 pM cTnI concentration, and upon the outbreak of acute myocardial infraction it can raise to 2 nM and levels at 20 nM for 6–8 days, therefore the achieved LoD covers most of the clinically relevant range. In addition, this system allows for the detection of troponin I using a single specific monoclonal antibody, which is highly beneficial in case of detection in real samples, where the protein has a complex form leading to hidden epitopes, thus paving the way towards a system that can improve early-stage screening of heart attacks.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.200871troponin isurface plasmonsingle monoclonal antibodybiosensor |
| spellingShingle | Anıl Bozdogan Reham F. El-Kased Vanessa Jungbluth Wolfgang Knoll Jakub Dostalek Amal Kasry Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody Royal Society Open Science troponin i surface plasmon single monoclonal antibody biosensor |
| title | Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody |
| title_full | Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody |
| title_fullStr | Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody |
| title_full_unstemmed | Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody |
| title_short | Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody |
| title_sort | development of a specific troponin i detection system with enhanced immune sensitivity using a single monoclonal antibody |
| topic | troponin i surface plasmon single monoclonal antibody biosensor |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.200871 |
| work_keys_str_mv | AT anılbozdogan developmentofaspecifictroponinidetectionsystemwithenhancedimmunesensitivityusingasinglemonoclonalantibody AT rehamfelkased developmentofaspecifictroponinidetectionsystemwithenhancedimmunesensitivityusingasinglemonoclonalantibody AT vanessajungbluth developmentofaspecifictroponinidetectionsystemwithenhancedimmunesensitivityusingasinglemonoclonalantibody AT wolfgangknoll developmentofaspecifictroponinidetectionsystemwithenhancedimmunesensitivityusingasinglemonoclonalantibody AT jakubdostalek developmentofaspecifictroponinidetectionsystemwithenhancedimmunesensitivityusingasinglemonoclonalantibody AT amalkasry developmentofaspecifictroponinidetectionsystemwithenhancedimmunesensitivityusingasinglemonoclonalantibody |