Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine
Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] plays a key role in the modulation of actin polymerization and vesicle trafficking. These processes seem to depend on the enrichment of PI(4,5)P2 in plasma membrane domains. Here, we show that PI(4,5)P2 does not form domains when in a fluid phospha...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2006-07-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520331965 |
| _version_ | 1818936702290362368 |
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| author | Faébio Fernandes Luiés M.S. Loura Alexander Fedorov Manuel Prieto |
| author_facet | Faébio Fernandes Luiés M.S. Loura Alexander Fedorov Manuel Prieto |
| author_sort | Faébio Fernandes |
| collection | DOAJ |
| description | Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] plays a key role in the modulation of actin polymerization and vesicle trafficking. These processes seem to depend on the enrichment of PI(4,5)P2 in plasma membrane domains. Here, we show that PI(4,5)P2 does not form domains when in a fluid phosphatidylcholine matrix in the pH range of 4.8–8.4. This finding is at variance with the spontaneous segregation of PI(4,5)P2 to domains as a mechanism for the compartmentalization of PI(4,5)P2 in the plasma membrane. Water/bilayer partition of PI(4,5)P2 is also shown to be dependent on the protonation state of the lipid. |
| first_indexed | 2024-12-20T05:40:16Z |
| format | Article |
| id | doaj.art-33d82e3ed074485b9ad6930e07189891 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T05:40:16Z |
| publishDate | 2006-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-33d82e3ed074485b9ad6930e071898912022-12-21T19:51:28ZengElsevierJournal of Lipid Research0022-22752006-07-0147715211525Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholineFaébio Fernandes0Luiés M.S. Loura1Alexander Fedorov2Manuel Prieto3Centro de Quiémica-Fiésica Molecular, Instituto Superior Teécnico, Lisbon, PortugalCentro de Quiémica-Fiésica Molecular, Instituto Superior Teécnico, Lisbon, Portugal; Centro de Quiémica e Departamento de Quiémica, Universidade de Eévora, Eévora, PortugalCentro de Quiémica-Fiésica Molecular, Instituto Superior Teécnico, Lisbon, PortugalCentro de Quiémica-Fiésica Molecular, Instituto Superior Teécnico, Lisbon, PortugalPhosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] plays a key role in the modulation of actin polymerization and vesicle trafficking. These processes seem to depend on the enrichment of PI(4,5)P2 in plasma membrane domains. Here, we show that PI(4,5)P2 does not form domains when in a fluid phosphatidylcholine matrix in the pH range of 4.8–8.4. This finding is at variance with the spontaneous segregation of PI(4,5)P2 to domains as a mechanism for the compartmentalization of PI(4,5)P2 in the plasma membrane. Water/bilayer partition of PI(4,5)P2 is also shown to be dependent on the protonation state of the lipid.http://www.sciencedirect.com/science/article/pii/S0022227520331965PIP2lipid domainsfluorescencefluorescence resonance energy transfer |
| spellingShingle | Faébio Fernandes Luiés M.S. Loura Alexander Fedorov Manuel Prieto Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine Journal of Lipid Research PIP2 lipid domains fluorescence fluorescence resonance energy transfer |
| title | Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine |
| title_full | Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine |
| title_fullStr | Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine |
| title_full_unstemmed | Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine |
| title_short | Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine |
| title_sort | absence of clustering of phosphatidylinositol 4 5 bisphosphate in fluid phosphatidylcholine |
| topic | PIP2 lipid domains fluorescence fluorescence resonance energy transfer |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520331965 |
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