Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to inc...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-08177-8 |