Effects of Persimmon (<i>Diospyros kaki</i> L. cv. Mopan) Polysaccharide and Their Carboxymethylated Derivatives on <i>Lactobacillus</i> Strains Proliferation and Gut Microbiota: A Comparative Study
Persimmon is a fruit that contains sugars, vitamins, phenolic compounds, and various other nutrients. The aim of this study was to explore the structure of carboxymethylated persimmon polysaccharide (CM-PFP) and its interaction with the human gut microbiota. Carboxymethyl modification of the persimm...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15730 |
| _version_ | 1797631776664846336 |
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| author | Xiaowei Shen Shanshan Xie Huixin Zhang Tao Wang Bolin Zhang Hongfei Zhao |
| author_facet | Xiaowei Shen Shanshan Xie Huixin Zhang Tao Wang Bolin Zhang Hongfei Zhao |
| author_sort | Xiaowei Shen |
| collection | DOAJ |
| description | Persimmon is a fruit that contains sugars, vitamins, phenolic compounds, and various other nutrients. The aim of this study was to explore the structure of carboxymethylated persimmon polysaccharide (CM-PFP) and its interaction with the human gut microbiota. Carboxymethyl modification of the persimmon polysaccharide (PFP) increased both the Mw and Mn, enhanced dispersion stability, and decreased thermal stability. Both PFP and CM-PFP promoted the proliferation of <i>Lactobacillus</i> while inhibiting the proliferation of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. In the simulated fecal fermentation, the pH of PFP- and CM-PFP-containing media decreased, the content of short-chain fatty acids increased, and the abundance of intestinal flora at the phylum and genus levels changed. The relative abundance of harmful intestinal bacteria was significantly reduced in both PFP and CM-PFP groups. Furthermore, it was found that CM-PFP was more easily metabolized than PFP, glucose, and fructo-oligosaccharide (FOS) and had a proliferation increase effect on <i>Lactobacillus</i>. Therefore, CM-PFP has a significant positive effect on both <i>Lactobacillus</i> proliferation and the human gut microbiota. |
| first_indexed | 2024-03-11T11:28:31Z |
| format | Article |
| id | doaj.art-3448334650dc4cbab78a43211e472e97 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:28:31Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3448334650dc4cbab78a43211e472e972023-11-10T15:05:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124211573010.3390/ijms242115730Effects of Persimmon (<i>Diospyros kaki</i> L. cv. Mopan) Polysaccharide and Their Carboxymethylated Derivatives on <i>Lactobacillus</i> Strains Proliferation and Gut Microbiota: A Comparative StudyXiaowei Shen0Shanshan Xie1Huixin Zhang2Tao Wang3Bolin Zhang4Hongfei Zhao5Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Science & Biotechnology, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Forest Food Processing and Safety, College of Biological Science & Biotechnology, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Forest Food Processing and Safety, College of Biological Science & Biotechnology, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Forest Food Processing and Safety, College of Biological Science & Biotechnology, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Forest Food Processing and Safety, College of Biological Science & Biotechnology, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Forest Food Processing and Safety, College of Biological Science & Biotechnology, Beijing Forestry University, Beijing 100083, ChinaPersimmon is a fruit that contains sugars, vitamins, phenolic compounds, and various other nutrients. The aim of this study was to explore the structure of carboxymethylated persimmon polysaccharide (CM-PFP) and its interaction with the human gut microbiota. Carboxymethyl modification of the persimmon polysaccharide (PFP) increased both the Mw and Mn, enhanced dispersion stability, and decreased thermal stability. Both PFP and CM-PFP promoted the proliferation of <i>Lactobacillus</i> while inhibiting the proliferation of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. In the simulated fecal fermentation, the pH of PFP- and CM-PFP-containing media decreased, the content of short-chain fatty acids increased, and the abundance of intestinal flora at the phylum and genus levels changed. The relative abundance of harmful intestinal bacteria was significantly reduced in both PFP and CM-PFP groups. Furthermore, it was found that CM-PFP was more easily metabolized than PFP, glucose, and fructo-oligosaccharide (FOS) and had a proliferation increase effect on <i>Lactobacillus</i>. Therefore, CM-PFP has a significant positive effect on both <i>Lactobacillus</i> proliferation and the human gut microbiota.https://www.mdpi.com/1422-0067/24/21/15730carboxymethyl modificationpersimmonpolysaccharides<i>Lactobacillus</i>gut microbiota |
| spellingShingle | Xiaowei Shen Shanshan Xie Huixin Zhang Tao Wang Bolin Zhang Hongfei Zhao Effects of Persimmon (<i>Diospyros kaki</i> L. cv. Mopan) Polysaccharide and Their Carboxymethylated Derivatives on <i>Lactobacillus</i> Strains Proliferation and Gut Microbiota: A Comparative Study International Journal of Molecular Sciences carboxymethyl modification persimmon polysaccharides <i>Lactobacillus</i> gut microbiota |
| title | Effects of Persimmon (<i>Diospyros kaki</i> L. cv. Mopan) Polysaccharide and Their Carboxymethylated Derivatives on <i>Lactobacillus</i> Strains Proliferation and Gut Microbiota: A Comparative Study |
| title_full | Effects of Persimmon (<i>Diospyros kaki</i> L. cv. Mopan) Polysaccharide and Their Carboxymethylated Derivatives on <i>Lactobacillus</i> Strains Proliferation and Gut Microbiota: A Comparative Study |
| title_fullStr | Effects of Persimmon (<i>Diospyros kaki</i> L. cv. Mopan) Polysaccharide and Their Carboxymethylated Derivatives on <i>Lactobacillus</i> Strains Proliferation and Gut Microbiota: A Comparative Study |
| title_full_unstemmed | Effects of Persimmon (<i>Diospyros kaki</i> L. cv. Mopan) Polysaccharide and Their Carboxymethylated Derivatives on <i>Lactobacillus</i> Strains Proliferation and Gut Microbiota: A Comparative Study |
| title_short | Effects of Persimmon (<i>Diospyros kaki</i> L. cv. Mopan) Polysaccharide and Their Carboxymethylated Derivatives on <i>Lactobacillus</i> Strains Proliferation and Gut Microbiota: A Comparative Study |
| title_sort | effects of persimmon i diospyros kaki i l cv mopan polysaccharide and their carboxymethylated derivatives on i lactobacillus i strains proliferation and gut microbiota a comparative study |
| topic | carboxymethyl modification persimmon polysaccharides <i>Lactobacillus</i> gut microbiota |
| url | https://www.mdpi.com/1422-0067/24/21/15730 |
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