Purification of rat pancreatic lipase

A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm. DEAE-cellulose chromatography, precipitation of amylase as the amy...

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Bibliographic Details
Main Author: Lewis I. Gidez
Format: Article
Language:English
Published: Elsevier 1968-11-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520426951