Analyzing siRNA Concentration, Complexation and Stability in Cationic Dendriplexes by Stem-Loop Reverse Transcription-qPCR

Analyzing siRNA Concentration, Complexation and Stability in Cationic Dendriplexes by Stem-Loop Reverse Transcription-qPCR

RNA interference (RNAi) is a powerful therapeutic approach for messenger RNA (mRNA) level regulation in human cells. RNAi can be triggered by small interfering RNAs (siRNAs) which are delivered by non-viral carriers, e.g., dendriplexes. siRNA quantification inside carriers is essential in drug deliv...

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Bibliographic Details
Main Authors: Maximilian Neugebauer, Clara E. Grundmann, Michael Lehnert, Felix von Stetten, Susanna M. Früh, Regine Süss
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:Pharmaceutics
Subjects:
reverse transcription real-time polymerase chain reaction (RT-qPCR)
stem-loop primer
small interfering RNA (siRNA)
drug delivery
dendriplexes
nitrogen-to-phosphate (N/P) ratio
Online Access:https://www.mdpi.com/1999-4923/14/7/1348

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https://www.mdpi.com/1999-4923/14/7/1348

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