Baicalein Attenuates Pyroptosis and Endoplasmic Reticulum Stress Following Spinal Cord Ischemia-Reperfusion Injury via Autophagy Enhancement
BackgroundSpinal cord ischemia-reperfusion injury (SCIRI) is the main complication after the repair of a complex thoracoabdominal aortic aneurysm. Many clinical treatments are not ideal due to the complex pathophysiological process of this injury. Baicalein (BA), a component derived from the roots o...
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2020.01076/full |
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| author | Chenyu Wu Chenyu Wu Hui Xu Hui Xu Jiafeng Li Jiafeng Li Xinli Hu Xinli Hu Xingyu Wang Xingyu Wang Yijia Huang Yijia Huang Yao Li Yao Li Sunren Sheng Sunren Sheng Yongli Wang Huazi Xu Huazi Xu Wenfei Ni Wenfei Ni Kailiang Zhou Kailiang Zhou |
| author_facet | Chenyu Wu Chenyu Wu Hui Xu Hui Xu Jiafeng Li Jiafeng Li Xinli Hu Xinli Hu Xingyu Wang Xingyu Wang Yijia Huang Yijia Huang Yao Li Yao Li Sunren Sheng Sunren Sheng Yongli Wang Huazi Xu Huazi Xu Wenfei Ni Wenfei Ni Kailiang Zhou Kailiang Zhou |
| author_sort | Chenyu Wu |
| collection | DOAJ |
| description | BackgroundSpinal cord ischemia-reperfusion injury (SCIRI) is the main complication after the repair of a complex thoracoabdominal aortic aneurysm. Many clinical treatments are not ideal due to the complex pathophysiological process of this injury. Baicalein (BA), a component derived from the roots of the herb Scutellaria baicalensis, may contribute to the successful treatment of ischemia/reperfusion injury.PurposeIn the present study, the effects of BA on spinal cord ischemia-reperfusion injury and the underlying mechanisms were assessed.Materials and MethodsSpinal cord ischemia was induced in C57BL/6 mice by blocking the aortic arch. Fifty-five mice were then randomly divided into four groups: Sham, SCIR+Vehicle, SCIR+BA, and SCIR+BA +3MA groups. At 0 and 24 h pre-SCIRI and at 24 h and 7 days post-SCIRI, evaluations with the Basso mouse scale (BMS) were performed. On postoperative 24 h, the spinal cord was harvested to assess pyroptosis, endoplasmic reticulum stress mediated apoptosis and autophagy.ResultsBA enhanced the functional recovery of spinal cord ischemia-reperfusion injury. In addition, BA attenuated pyroptosis, alleviated endoplasmic reticulum stress-mediated apoptosis, and activated autophagy. However, the effects of BA on the functional recovery of SCIRI, pyroptosis and endoplasmic reticulum stress-mediated apoptosis were reversed by the inhibition of autophagy.ConclusionsIn general, our findings revealed that BA enhances the functional recovery of spinal cord ischemia-reperfusion injury by dampening pyroptosis and alleviating endoplasmic reticulum stress-mediated apoptosis, which are mediated by the activation of autophagy. |
| first_indexed | 2024-12-11T14:21:55Z |
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| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T14:21:55Z |
| publishDate | 2020-07-01 |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-349cd11488c54e10ba95d31cc24653eb2022-12-22T01:02:52ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-07-011110.3389/fphar.2020.01076550477Baicalein Attenuates Pyroptosis and Endoplasmic Reticulum Stress Following Spinal Cord Ischemia-Reperfusion Injury via Autophagy EnhancementChenyu Wu0Chenyu Wu1Hui Xu2Hui Xu3Jiafeng Li4Jiafeng Li5Xinli Hu6Xinli Hu7Xingyu Wang8Xingyu Wang9Yijia Huang10Yijia Huang11Yao Li12Yao Li13Sunren Sheng14Sunren Sheng15Yongli Wang16Huazi Xu17Huazi Xu18Wenfei Ni19Wenfei Ni20Kailiang Zhou21Kailiang Zhou22Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, Huzhou Central Hospital, Huzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, ChinaBackgroundSpinal cord ischemia-reperfusion injury (SCIRI) is the main complication after the repair of a complex thoracoabdominal aortic aneurysm. Many clinical treatments are not ideal due to the complex pathophysiological process of this injury. Baicalein (BA), a component derived from the roots of the herb Scutellaria baicalensis, may contribute to the successful treatment of ischemia/reperfusion injury.PurposeIn the present study, the effects of BA on spinal cord ischemia-reperfusion injury and the underlying mechanisms were assessed.Materials and MethodsSpinal cord ischemia was induced in C57BL/6 mice by blocking the aortic arch. Fifty-five mice were then randomly divided into four groups: Sham, SCIR+Vehicle, SCIR+BA, and SCIR+BA +3MA groups. At 0 and 24 h pre-SCIRI and at 24 h and 7 days post-SCIRI, evaluations with the Basso mouse scale (BMS) were performed. On postoperative 24 h, the spinal cord was harvested to assess pyroptosis, endoplasmic reticulum stress mediated apoptosis and autophagy.ResultsBA enhanced the functional recovery of spinal cord ischemia-reperfusion injury. In addition, BA attenuated pyroptosis, alleviated endoplasmic reticulum stress-mediated apoptosis, and activated autophagy. However, the effects of BA on the functional recovery of SCIRI, pyroptosis and endoplasmic reticulum stress-mediated apoptosis were reversed by the inhibition of autophagy.ConclusionsIn general, our findings revealed that BA enhances the functional recovery of spinal cord ischemia-reperfusion injury by dampening pyroptosis and alleviating endoplasmic reticulum stress-mediated apoptosis, which are mediated by the activation of autophagy.https://www.frontiersin.org/article/10.3389/fphar.2020.01076/fullbaicaleinspinal cord ischemia-reperfusion injuryautophagypyroptosisendoplasmic reticulum stress |
| spellingShingle | Chenyu Wu Chenyu Wu Hui Xu Hui Xu Jiafeng Li Jiafeng Li Xinli Hu Xinli Hu Xingyu Wang Xingyu Wang Yijia Huang Yijia Huang Yao Li Yao Li Sunren Sheng Sunren Sheng Yongli Wang Huazi Xu Huazi Xu Wenfei Ni Wenfei Ni Kailiang Zhou Kailiang Zhou Baicalein Attenuates Pyroptosis and Endoplasmic Reticulum Stress Following Spinal Cord Ischemia-Reperfusion Injury via Autophagy Enhancement Frontiers in Pharmacology baicalein spinal cord ischemia-reperfusion injury autophagy pyroptosis endoplasmic reticulum stress |
| title | Baicalein Attenuates Pyroptosis and Endoplasmic Reticulum Stress Following Spinal Cord Ischemia-Reperfusion Injury via Autophagy Enhancement |
| title_full | Baicalein Attenuates Pyroptosis and Endoplasmic Reticulum Stress Following Spinal Cord Ischemia-Reperfusion Injury via Autophagy Enhancement |
| title_fullStr | Baicalein Attenuates Pyroptosis and Endoplasmic Reticulum Stress Following Spinal Cord Ischemia-Reperfusion Injury via Autophagy Enhancement |
| title_full_unstemmed | Baicalein Attenuates Pyroptosis and Endoplasmic Reticulum Stress Following Spinal Cord Ischemia-Reperfusion Injury via Autophagy Enhancement |
| title_short | Baicalein Attenuates Pyroptosis and Endoplasmic Reticulum Stress Following Spinal Cord Ischemia-Reperfusion Injury via Autophagy Enhancement |
| title_sort | baicalein attenuates pyroptosis and endoplasmic reticulum stress following spinal cord ischemia reperfusion injury via autophagy enhancement |
| topic | baicalein spinal cord ischemia-reperfusion injury autophagy pyroptosis endoplasmic reticulum stress |
| url | https://www.frontiersin.org/article/10.3389/fphar.2020.01076/full |
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