Tracking the growth fate of single cells and isolating slow-growing cells in human colorectal cancer organoids

Summary: Patient-derived tumor organoids are three-dimensionally cultured cancer cells that enable a suitable platform for studying heterogeneity and plasticity of cancer. We present a protocol for tracking the growth fate of single cells and isolating slow-growing cells in human colorectal cancer o...

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Bibliographic Details
Main Authors: Roberto Coppo, Jumpei Kondo, Kunishige Onuma, Masahiro Inoue
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003623