Optical tweezers assisted imaging of the Z-ring in Escherichia coli: measuring its radial width
Using single-beam, oscillating optical tweezers we can trap and rotate rod-shaped bacterial cells with respect to the optical axis. This technique allows imaging fluorescently labeled three-dimensional sub-cellular structures from different, optimized viewpoints. To illustrate our method we measure...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
IOP Publishing
2014-01-01
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Series: | New Journal of Physics |
Online Access: | https://doi.org/10.1088/1367-2630/16/1/013043 |