FRAP analysis of chromatin looseness in mouse zygotes that allows full-term development.

FRAP analysis of chromatin looseness in mouse zygotes that allows full-term development.

Chromatin looseness, which can be analyzed by fluorescence recovery after photobleaching (FRAP) using eGFP-tagged core histone proteins, is an important index of the differentiation potential of blastomere cells and embryonic stem cells. Whether chromatin looseness is a reliable index of the develop...

Full description

Bibliographic Details
Main Authors:	Masatoshi Ooga, Teruhiko Wakayama
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2017-01-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC5440048?pdf=render

Similar Items

Parental competition for the regulators of chromatin dynamics in mouse zygotes
by: Masatoshi Ooga, et al.
Published: (2022-07-01)

Production of mouse offspring from zygotes fertilized with freeze-dried spermatids
by: Sayaka Wakayama, et al.
Published: (2022-11-01)

Mailing viable mouse freeze-dried spermatozoa on postcards
by: Daiyu Ito, et al.
Published: (2021-08-01)

Improvement of a twice collection method of mouse oocytes by surgical operation
by: Rei INOUE, et al.
Published: (2020-06-01)

Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method
by: Yasuyuki Kikuchi, et al.
Published: (2023-11-01)

Optimised CO2-containing medium for in vitro culture and transportation of mouse preimplantation embryos without CO2 incubator.
by: Yasuyuki Kikuchi, et al.
Published: (2021-01-01)

Production of offspring from vacuum-dried mouse spermatozoa and assessing the effect of drying conditions on sperm DNA and embryo development
by: Natsuki USHIGOME, et al.
Published: (2022-06-01)

Single nucleolus precursor body formation in the pronucleus of mouse zygotes and SCNT embryos.
by: Hirohisa Kyogoku, et al.
Published: (2018-01-01)

A novel, simplified method to prepare and preserve freeze-dried mouse sperm in plastic microtubes
by: Li Ly YANG, et al.
Published: (2023-06-01)

Protocol for detecting chromatin dynamics and screening chromatin relaxer by FRAP assay
by: Qi Long, et al.
Published: (2021-09-01)

Production of mouse offspring from inactivated spermatozoa using horse PLCζ mRNA
by: Yunosuke YAMAMOTO, et al.
Published: (2019-12-01)

Mouse in vivo-derived late 2-cell embryos have higher developmental competence after high osmolality vitrification and −80°C preservation than IVF or ICSI embryos
by: Erika HAYASHI, et al.
Published: (2022-01-01)

Effect of trehalose on the preservation of freeze-dried mice spermatozoa at room temperature
by: Daiyu ITO, et al.
Published: (2019-05-01)

Evaluation of pulsed-FRAP and conventional-FRAP for determination of protein mobility in prokaryotic cells.
by: Jacek T Mika, et al.
Published: (2011-01-01)

The ferric reducing ability of plasma (FRAP) as a measure of " Antioxidant Power": The FRAP Assay/
by: Benzie, Iris F. F., et al.

Analysis of membrane-localized binding kinetics with FRAP.
by: Dushek, O, et al.
Published: (2008)

Mouse zygote-specific proteasome assembly chaperone important for maternal-to-zygotic transition
by: Seung-Wook Shin, et al.
Published: (2012-11-01)

FRAP analysis: accounting for bleaching during image capture.
by: Jun Wu, et al.
Published: (2012-01-01)

GAF is essential for zygotic genome activation and chromatin accessibility in the early Drosophila embryo
by: Marissa M Gaskill, et al.
Published: (2021-03-01)

Cortical softening elicits zygotic contractility during mouse preimplantation development.
by: Özge Özgüç, et al.
Published: (2022-03-01)

Single-nucleus Hi-C reveals unique chromatin reorganization at oocyte-to-zygote transition
by: Flyamer, Ilya M., et al.
Published: (2017)

Improving parameter estimation for cell surface FRAP data.
by: Dushek, O, et al.
Published: (2008)

Measuring diffusion and binding kinetics by contact area FRAP.
by: Tolentino, T, et al.
Published: (2008)

Photoswitching-free FRAP analysis with a genetically encoded fluorescent tag.
by: Tatsuya Morisaki, et al.
Published: (2014-01-01)

Dynamic shapes of the zygote and two-cell mouse and human
by: Graham, CF, et al.
Published: (2021)

Effective and precise adenine base editing in mouse zygotes
by: Puping Liang, et al.
Published: (2018-07-01)

Increasing knock-in efficiency in mouse zygotes by transient hypothermia
by: Bouchareb, A, et al.
Published: (2024)

Dynamic shapes of the zygote and two-cell mouse and human
by: Chris F. Graham, et al.
Published: (2021-12-01)

Asymmetric Reprogramming Capacity of Parental Pronuclei in Mouse Zygotes
by: Wenqiang Liu, et al.
Published: (2014-03-01)

Transcriptome Analysis of Zygotic Induction During Conjugative Transfer of Plasmid RP4
by: Masatoshi Miyakoshi, et al.
Published: (2020-06-01)

Automated microinjection of recombinant BCL-X into mouse zygotes enhances embryo development.
by: Xinyu Liu, et al.
Published: (2011-01-01)

A coupled diffusion-kinetics model for analysis of contact-area FRAP experiment.
by: Wu, J, et al.
Published: (2008)

FRAP analysis reveals stabilization of adhesion structures in the epidermis compared to cultured keratinocytes.
by: Henry P Foote, et al.
Published: (2013-01-01)

Confocal FRAP studies to monitor protein dynamics in live cells
by: Tilley, L, et al.
Published: (2002)

Approach to Optimization of FRAP Methodology for Studies Based on Selected Monoterpenes
by: Karolina A. Wojtunik-Kulesza
Published: (2020-11-01)

Antioxidant Activity of some Medicinal Species using FRAP Assay
by: AR Gohari, et al.
Published: (2011-03-01)

FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments.
by: Frédéric Pincet, et al.
Published: (2016-01-01)

An investigation of the origin and significance of bilateral symmetry of the pronuclear zygote in the mouse.
by: Gardner, R, et al.
Published: (2006)

5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote.
by: Juliette Salvaing, et al.
Published: (2012-01-01)

Subcellular distribution of mitochondrial ribosomal RNA in the mouse oocyte and zygote.
by: Youichirou Ninomiya, et al.
Published: (2007-11-01)