Non-selective cation channels mediate chloroquine-induced relaxation in precontracted mouse airway smooth muscle.
Bitter tastants can induce relaxation in precontracted airway smooth muscle by activating big-conductance potassium channels (BKs) or by inactivating voltage-dependent L-type Ca2+ channels (VDLCCs). In this study, a new pathway for bitter tastant-induced relaxation was defined and investigated. We f...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4081631?pdf=render |
| _version_ | 1818925112222547968 |
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| author | Ting Zhang Xiao-Jing Luo Wen-Bo Sai Meng-Fei Yu Wen-Er Li Yun-Fei Ma Weiwei Chen Kui Zhai Gangjian Qin Donglin Guo Yun-Min Zheng Yong-Xiao Wang Jin-Hua Shen Guangju Ji Qing-Hua Liu |
| author_facet | Ting Zhang Xiao-Jing Luo Wen-Bo Sai Meng-Fei Yu Wen-Er Li Yun-Fei Ma Weiwei Chen Kui Zhai Gangjian Qin Donglin Guo Yun-Min Zheng Yong-Xiao Wang Jin-Hua Shen Guangju Ji Qing-Hua Liu |
| author_sort | Ting Zhang |
| collection | DOAJ |
| description | Bitter tastants can induce relaxation in precontracted airway smooth muscle by activating big-conductance potassium channels (BKs) or by inactivating voltage-dependent L-type Ca2+ channels (VDLCCs). In this study, a new pathway for bitter tastant-induced relaxation was defined and investigated. We found nifedipine-insensitive and bitter tastant chloroquine-sensitive relaxation in epithelium-denuded mouse tracheal rings (TRs) precontracted with acetylcholine (ACH). In the presence of nifedipine (10 µM), ACH induced cytosolic Ca2+ elevation and cell shortening in single airway smooth muscle cells (ASMCs), and these changes were inhibited by chloroquine. In TRs, ACH triggered a transient contraction under Ca2+-free conditions, and, following a restoration of Ca2+, a strong contraction occurred, which was inhibited by chloroquine. Moreover, the ACH-activated whole-cell and single channel currents of non-selective cation channels (NSCCs) were blocked by chloroquine. Pyrazole 3 (Pyr3), an inhibitor of transient receptor potential C3 (TRPC3) channels, partially inhibited ACH-induced contraction, intracellular Ca2+ elevation, and NSCC currents. These results demonstrate that NSCCs play a role in bitter tastant-induced relaxation in precontracted airway smooth muscle. |
| first_indexed | 2024-12-20T02:36:02Z |
| format | Article |
| id | doaj.art-351fe5a6d38c43e5850b23c94e301efb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T02:36:02Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-351fe5a6d38c43e5850b23c94e301efb2022-12-21T19:56:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10157810.1371/journal.pone.0101578Non-selective cation channels mediate chloroquine-induced relaxation in precontracted mouse airway smooth muscle.Ting ZhangXiao-Jing LuoWen-Bo SaiMeng-Fei YuWen-Er LiYun-Fei MaWeiwei ChenKui ZhaiGangjian QinDonglin GuoYun-Min ZhengYong-Xiao WangJin-Hua ShenGuangju JiQing-Hua LiuBitter tastants can induce relaxation in precontracted airway smooth muscle by activating big-conductance potassium channels (BKs) or by inactivating voltage-dependent L-type Ca2+ channels (VDLCCs). In this study, a new pathway for bitter tastant-induced relaxation was defined and investigated. We found nifedipine-insensitive and bitter tastant chloroquine-sensitive relaxation in epithelium-denuded mouse tracheal rings (TRs) precontracted with acetylcholine (ACH). In the presence of nifedipine (10 µM), ACH induced cytosolic Ca2+ elevation and cell shortening in single airway smooth muscle cells (ASMCs), and these changes were inhibited by chloroquine. In TRs, ACH triggered a transient contraction under Ca2+-free conditions, and, following a restoration of Ca2+, a strong contraction occurred, which was inhibited by chloroquine. Moreover, the ACH-activated whole-cell and single channel currents of non-selective cation channels (NSCCs) were blocked by chloroquine. Pyrazole 3 (Pyr3), an inhibitor of transient receptor potential C3 (TRPC3) channels, partially inhibited ACH-induced contraction, intracellular Ca2+ elevation, and NSCC currents. These results demonstrate that NSCCs play a role in bitter tastant-induced relaxation in precontracted airway smooth muscle.http://europepmc.org/articles/PMC4081631?pdf=render |
| spellingShingle | Ting Zhang Xiao-Jing Luo Wen-Bo Sai Meng-Fei Yu Wen-Er Li Yun-Fei Ma Weiwei Chen Kui Zhai Gangjian Qin Donglin Guo Yun-Min Zheng Yong-Xiao Wang Jin-Hua Shen Guangju Ji Qing-Hua Liu Non-selective cation channels mediate chloroquine-induced relaxation in precontracted mouse airway smooth muscle. PLoS ONE |
| title | Non-selective cation channels mediate chloroquine-induced relaxation in precontracted mouse airway smooth muscle. |
| title_full | Non-selective cation channels mediate chloroquine-induced relaxation in precontracted mouse airway smooth muscle. |
| title_fullStr | Non-selective cation channels mediate chloroquine-induced relaxation in precontracted mouse airway smooth muscle. |
| title_full_unstemmed | Non-selective cation channels mediate chloroquine-induced relaxation in precontracted mouse airway smooth muscle. |
| title_short | Non-selective cation channels mediate chloroquine-induced relaxation in precontracted mouse airway smooth muscle. |
| title_sort | non selective cation channels mediate chloroquine induced relaxation in precontracted mouse airway smooth muscle |
| url | http://europepmc.org/articles/PMC4081631?pdf=render |
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