Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity?
Soybean (Glycine max) produces a class of phenylalanine (Phe) derived specialized metabolites, isoflavonoids. Isoflavonoids are unique to legumes and are involved in defense responses in planta, and they are also necessary for nodule formation with nitrogen-fixing bacteria. Since Phe is a precursor...
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| Format: | Article |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1307489/full |
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| author | Emily J. Clayton Emily J. Clayton Nishat S. Islam Kelsey Pannunzio Kelsey Pannunzio Kuflom Kuflu Ramtin Sirjani Ramtin Sirjani Susanne E. Kohalmi Sangeeta Dhaubhadel Sangeeta Dhaubhadel |
| author_facet | Emily J. Clayton Emily J. Clayton Nishat S. Islam Kelsey Pannunzio Kelsey Pannunzio Kuflom Kuflu Ramtin Sirjani Ramtin Sirjani Susanne E. Kohalmi Sangeeta Dhaubhadel Sangeeta Dhaubhadel |
| author_sort | Emily J. Clayton |
| collection | DOAJ |
| description | Soybean (Glycine max) produces a class of phenylalanine (Phe) derived specialized metabolites, isoflavonoids. Isoflavonoids are unique to legumes and are involved in defense responses in planta, and they are also necessary for nodule formation with nitrogen-fixing bacteria. Since Phe is a precursor of isoflavonoids, it stands to reason that the synthesis of Phe is coordinated with isoflavonoid production. Two putative AROGENATE DEHYDRATASE (ADT) isoforms were previously co-purified with the soybean isoflavonoid metabolon anchor ISOFLAVONE SYNTHASE2 (GmIFS2), however the GmADT family had not been characterized. Here, we present the identification of the nine member GmADT family. We determined that the GmADTs share sequences required for enzymatic activity and allosteric regulation with other characterized plant ADTs. Furthermore, the GmADTs are differentially expressed, and multiple members have dual substrate specificity, also acting as PREPHENATE DEHYDRATASES. All GmADT isoforms were detected in the stromules of chloroplasts, and they all interact with GmIFS2 in the cytosol. In addition, GmADT12A interacts with multiple other isoflavonoid metabolon members. These data substantiate the involvement of GmADT isoforms in the isoflavonoid metabolon. |
| first_indexed | 2024-03-08T12:03:26Z |
| format | Article |
| id | doaj.art-353e5892518c4c84a1cb8eea19be6ca4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T12:03:26Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-353e5892518c4c84a1cb8eea19be6ca42024-01-23T11:49:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-01-011510.3389/fpls.2024.13074891307489Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity?Emily J. Clayton0Emily J. Clayton1Nishat S. Islam2Kelsey Pannunzio3Kelsey Pannunzio4Kuflom Kuflu5Ramtin Sirjani6Ramtin Sirjani7Susanne E. Kohalmi8Sangeeta Dhaubhadel9Sangeeta Dhaubhadel10London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, CanadaDepartment of Biology, University of Western Ontario, London, ON, CanadaLondon Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, CanadaLondon Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, CanadaDepartment of Biology, University of Western Ontario, London, ON, CanadaLondon Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, CanadaLondon Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, CanadaDepartment of Biology, University of Western Ontario, London, ON, CanadaDepartment of Biology, University of Western Ontario, London, ON, CanadaLondon Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, CanadaDepartment of Biology, University of Western Ontario, London, ON, CanadaSoybean (Glycine max) produces a class of phenylalanine (Phe) derived specialized metabolites, isoflavonoids. Isoflavonoids are unique to legumes and are involved in defense responses in planta, and they are also necessary for nodule formation with nitrogen-fixing bacteria. Since Phe is a precursor of isoflavonoids, it stands to reason that the synthesis of Phe is coordinated with isoflavonoid production. Two putative AROGENATE DEHYDRATASE (ADT) isoforms were previously co-purified with the soybean isoflavonoid metabolon anchor ISOFLAVONE SYNTHASE2 (GmIFS2), however the GmADT family had not been characterized. Here, we present the identification of the nine member GmADT family. We determined that the GmADTs share sequences required for enzymatic activity and allosteric regulation with other characterized plant ADTs. Furthermore, the GmADTs are differentially expressed, and multiple members have dual substrate specificity, also acting as PREPHENATE DEHYDRATASES. All GmADT isoforms were detected in the stromules of chloroplasts, and they all interact with GmIFS2 in the cytosol. In addition, GmADT12A interacts with multiple other isoflavonoid metabolon members. These data substantiate the involvement of GmADT isoforms in the isoflavonoid metabolon.https://www.frontiersin.org/articles/10.3389/fpls.2024.1307489/fullsoybeanarogenate dehydrataseisoflavone synthasephenylalanineisoflavonoidmetabolon |
| spellingShingle | Emily J. Clayton Emily J. Clayton Nishat S. Islam Kelsey Pannunzio Kelsey Pannunzio Kuflom Kuflu Ramtin Sirjani Ramtin Sirjani Susanne E. Kohalmi Sangeeta Dhaubhadel Sangeeta Dhaubhadel Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity? Frontiers in Plant Science soybean arogenate dehydratase isoflavone synthase phenylalanine isoflavonoid metabolon |
| title | Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity? |
| title_full | Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity? |
| title_fullStr | Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity? |
| title_full_unstemmed | Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity? |
| title_short | Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity? |
| title_sort | soybean arogenate dehydratases gmadts involvement in the cytosolic isoflavonoid metabolon or trans organelle continuity |
| topic | soybean arogenate dehydratase isoflavone synthase phenylalanine isoflavonoid metabolon |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1307489/full |
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