Determining the effects of loss of function mutations in human cell lines

Determining the effects of loss of function mutations in human cell lines

Summary: Quantifying differences in the amount of protein and mRNA caused by missense mutations in a gene of interest can be challenging, especially when using patient-derived primary cells, which are intrinsically variable. In this protocol, we describe how to culture patient-derived lymphoblast an...

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Bibliographic Details
Main Authors: Nicola de Prisco, Salvatore Botta, Winston Lee, Sarallah Rezazadeh, Alexei Chemiakine, Vincenzo A. Gennarino
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Genetics
Model Organisms
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001125

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http://www.sciencedirect.com/science/article/pii/S2666166722001125

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