Antimicrobial and Antibiofilm Effect of ε-Polylysine against <i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> in Tryptic Soy Broth and Chicken Juice
ε-Polylysine (ε-PL) is a safe food additive that is used in the food industry globally. This study evaluated the antimicrobial and antibiofilm activity of antibacterial peptides (ε-PL) against food poisoning pathogens detected in chicken (<i>Salmonella</i> Enteritidis, <i>Listeria...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/9/2211 |
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| author | Do-Un Lee Yeong Jin Park Hwan Hee Yu Suk-Chae Jung Jung-Hee Park Dae-Hee Lee Na-Kyoung Lee Hyun-Dong Paik |
| author_facet | Do-Un Lee Yeong Jin Park Hwan Hee Yu Suk-Chae Jung Jung-Hee Park Dae-Hee Lee Na-Kyoung Lee Hyun-Dong Paik |
| author_sort | Do-Un Lee |
| collection | DOAJ |
| description | ε-Polylysine (ε-PL) is a safe food additive that is used in the food industry globally. This study evaluated the antimicrobial and antibiofilm activity of antibacterial peptides (ε-PL) against food poisoning pathogens detected in chicken (<i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i>). The results showed that minimum inhibitory concentrations (MICs) ranged between 0.031–1.0 mg/mL, although most bacterial groups (75%) showed MICs of 1.0 mg/mL. The reduction in the cell viability of pathogens due to ε-PL depended on the time and concentration, and 1/2 × MIC of ε-PL killed 99.99% of pathogens after 10 h of incubation. To confirm biofilm inhibition and degradation effects, crystal violet assay and confocal laser scanning microscopy (CLSM) were used. The biofilm formation rates of four bacterial groups (<i>Salmonella</i>, <i>Listeria</i>, <i>E. coli</i>, and multi-species bacteria) were 10.36%, 9.10%, 17.44%, and 21.37% at 1/2 × MIC of ε-PL, respectively. Additionally, when observed under a CLSM, ε-PL was found to induce biofilm destruction and bacterial cytotoxicity. These results demonstrated that ε-PL has the potential to be used as an antibiotic and antibiofilm material for chicken meat processing. |
| first_indexed | 2024-03-10T07:40:25Z |
| format | Article |
| id | doaj.art-357968324f394bb49bcbe8c7efeccbcb |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T07:40:25Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-357968324f394bb49bcbe8c7efeccbcb2023-11-22T13:06:00ZengMDPI AGFoods2304-81582021-09-01109221110.3390/foods10092211Antimicrobial and Antibiofilm Effect of ε-Polylysine against <i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> in Tryptic Soy Broth and Chicken JuiceDo-Un Lee0Yeong Jin Park1Hwan Hee Yu2Suk-Chae Jung3Jung-Hee Park4Dae-Hee Lee5Na-Kyoung Lee6Hyun-Dong Paik7Department of Food Science and Biotechnology of Animal Resource, Konkuk University, Seoul 05029, KoreaDepartment of Food Science and Biotechnology of Animal Resource, Konkuk University, Seoul 05029, KoreaDepartment of Food Science and Biotechnology of Animal Resource, Konkuk University, Seoul 05029, KoreaSempio Fermentation Research Center, Sempio Foods Company, Cheongju 28156, KoreaSempio Fermentation Research Center, Sempio Foods Company, Cheongju 28156, KoreaSempio Fermentation Research Center, Sempio Foods Company, Cheongju 28156, KoreaDepartment of Food Science and Biotechnology of Animal Resource, Konkuk University, Seoul 05029, KoreaDepartment of Food Science and Biotechnology of Animal Resource, Konkuk University, Seoul 05029, Koreaε-Polylysine (ε-PL) is a safe food additive that is used in the food industry globally. This study evaluated the antimicrobial and antibiofilm activity of antibacterial peptides (ε-PL) against food poisoning pathogens detected in chicken (<i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i>). The results showed that minimum inhibitory concentrations (MICs) ranged between 0.031–1.0 mg/mL, although most bacterial groups (75%) showed MICs of 1.0 mg/mL. The reduction in the cell viability of pathogens due to ε-PL depended on the time and concentration, and 1/2 × MIC of ε-PL killed 99.99% of pathogens after 10 h of incubation. To confirm biofilm inhibition and degradation effects, crystal violet assay and confocal laser scanning microscopy (CLSM) were used. The biofilm formation rates of four bacterial groups (<i>Salmonella</i>, <i>Listeria</i>, <i>E. coli</i>, and multi-species bacteria) were 10.36%, 9.10%, 17.44%, and 21.37% at 1/2 × MIC of ε-PL, respectively. Additionally, when observed under a CLSM, ε-PL was found to induce biofilm destruction and bacterial cytotoxicity. These results demonstrated that ε-PL has the potential to be used as an antibiotic and antibiofilm material for chicken meat processing.https://www.mdpi.com/2304-8158/10/9/2211ε-polylysine<i>Salmonella</i> Enteritidis<i>Listeria monocytogenes</i><i>Escherichia coli</i>antimicrobial effectantibiofilm |
| spellingShingle | Do-Un Lee Yeong Jin Park Hwan Hee Yu Suk-Chae Jung Jung-Hee Park Dae-Hee Lee Na-Kyoung Lee Hyun-Dong Paik Antimicrobial and Antibiofilm Effect of ε-Polylysine against <i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> in Tryptic Soy Broth and Chicken Juice Foods ε-polylysine <i>Salmonella</i> Enteritidis <i>Listeria monocytogenes</i> <i>Escherichia coli</i> antimicrobial effect antibiofilm |
| title | Antimicrobial and Antibiofilm Effect of ε-Polylysine against <i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> in Tryptic Soy Broth and Chicken Juice |
| title_full | Antimicrobial and Antibiofilm Effect of ε-Polylysine against <i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> in Tryptic Soy Broth and Chicken Juice |
| title_fullStr | Antimicrobial and Antibiofilm Effect of ε-Polylysine against <i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> in Tryptic Soy Broth and Chicken Juice |
| title_full_unstemmed | Antimicrobial and Antibiofilm Effect of ε-Polylysine against <i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> in Tryptic Soy Broth and Chicken Juice |
| title_short | Antimicrobial and Antibiofilm Effect of ε-Polylysine against <i>Salmonella</i> Enteritidis, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> in Tryptic Soy Broth and Chicken Juice |
| title_sort | antimicrobial and antibiofilm effect of ε polylysine against i salmonella i enteritidis i listeria monocytogenes i and i escherichia coli i in tryptic soy broth and chicken juice |
| topic | ε-polylysine <i>Salmonella</i> Enteritidis <i>Listeria monocytogenes</i> <i>Escherichia coli</i> antimicrobial effect antibiofilm |
| url | https://www.mdpi.com/2304-8158/10/9/2211 |
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