Construction and functional verification of size-reduced plasmids based on TMP resistance gene dfrB10

Construction and functional verification of size-reduced plasmids based on TMP resistance gene dfrB10

ABSTRACT Antibiotic resistance screening is crucial in the construction of recombinant plasmids. The dfrB10 gene, a short-size trimethoprim (TMP)-resistance type B dihydrofolate reductase gene, is a promising antibiotic drug screening marker for plasmid construction in molecular genetic research. Cu...

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Bibliographic Details
Main Authors: Chun-cao Li, Rui Hu, Xiu-min Hua, Yi-xuan Ni, Lu Ge, Lu Zhang, Wen Yu, Ni-xin Hao, Hui Xia, Qiang Fang, Zhi-yong Tao
Format: Article
Language:English
Published: American Society for Microbiology 2023-12-01
Series:Microbiology Spectrum
Subjects:
TMP
dfrB10
drug selection marker
plasmid
vector
Online Access:https://journals.asm.org/doi/10.1128/spectrum.01206-23

Internet

https://journals.asm.org/doi/10.1128/spectrum.01206-23

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