Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H<sub>2</sub>O<sub>2</sub>
Resveratrol is a polyphenolic compound with anti-oxidation effects. The mechanisms underlying the antioxidant effects of resveratrol in duck intestinal epithelial cells remain unclear. The protective effects of resveratrol against oxidative stress induced by H<sub>2</sub>O<sub>2<...
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| author | Ning Zhou Yong Tian Hongzhi Wu Yongqing Cao Ruiqing Li Kang Zou Wenwu Xu Lizhi Lu |
| author_facet | Ning Zhou Yong Tian Hongzhi Wu Yongqing Cao Ruiqing Li Kang Zou Wenwu Xu Lizhi Lu |
| author_sort | Ning Zhou |
| collection | DOAJ |
| description | Resveratrol is a polyphenolic compound with anti-oxidation effects. The mechanisms underlying the antioxidant effects of resveratrol in duck intestinal epithelial cells remain unclear. The protective effects of resveratrol against oxidative stress induced by H<sub>2</sub>O<sub>2</sub> on immortalized duck intestinal epithelial cells (IDECs) were investigated. IDECs were established by transferring the lentivirus-mediated simian virus 40 large T (<i>SV40T</i>) gene into small intestinal epithelial cells derived from duck embryos. IDECs were morphologically indistinguishable from the primary intestinal epithelial cells. The marker protein cytokeratin 18 (CK18) was also detected in the cultured cells. We found that resveratrol significantly increased the cell viability and activity of catalase and decreased the level of intracellular reactive oxygen species and malondialdehyde, as well as the apoptosis rate induced by H<sub>2</sub>O<sub>2</sub> (<i>p</i> < 0.05). Resveratrol up-regulated the expression of NRF2, p-NRF2, p-AKT, and p-P38 proteins and decreased the levels of cleaved caspase-3 and cleaved caspase-9 and the ratio of Bax to Bcl-2 in H<sub>2</sub>O<sub>2</sub>-induced IDECs (<i>p</i> < 0.05). Our findings revealed that resveratrol might alleviate oxidative stress by the PI3K/AKT and P38 MAPK signal pathways and inhibit apoptosis by altering the levels of cleaved caspase-3, cleaved caspase-9, Bax, and Bcl-2 in IDECs exposed to H<sub>2</sub>O<sub>2</sub>. |
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| language | English |
| last_indexed | 2024-03-10T01:03:24Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-35a324dbc2cc48d78f7529345a30be3f2023-11-23T14:30:32ZengMDPI AGMolecules1420-30492022-05-012711354210.3390/molecules27113542Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H<sub>2</sub>O<sub>2</sub>Ning Zhou0Yong Tian1Hongzhi Wu2Yongqing Cao3Ruiqing Li4Kang Zou5Wenwu Xu6Lizhi Lu7State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, ChinaTropical Crop Genetic Resource Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, ChinaResveratrol is a polyphenolic compound with anti-oxidation effects. The mechanisms underlying the antioxidant effects of resveratrol in duck intestinal epithelial cells remain unclear. The protective effects of resveratrol against oxidative stress induced by H<sub>2</sub>O<sub>2</sub> on immortalized duck intestinal epithelial cells (IDECs) were investigated. IDECs were established by transferring the lentivirus-mediated simian virus 40 large T (<i>SV40T</i>) gene into small intestinal epithelial cells derived from duck embryos. IDECs were morphologically indistinguishable from the primary intestinal epithelial cells. The marker protein cytokeratin 18 (CK18) was also detected in the cultured cells. We found that resveratrol significantly increased the cell viability and activity of catalase and decreased the level of intracellular reactive oxygen species and malondialdehyde, as well as the apoptosis rate induced by H<sub>2</sub>O<sub>2</sub> (<i>p</i> < 0.05). Resveratrol up-regulated the expression of NRF2, p-NRF2, p-AKT, and p-P38 proteins and decreased the levels of cleaved caspase-3 and cleaved caspase-9 and the ratio of Bax to Bcl-2 in H<sub>2</sub>O<sub>2</sub>-induced IDECs (<i>p</i> < 0.05). Our findings revealed that resveratrol might alleviate oxidative stress by the PI3K/AKT and P38 MAPK signal pathways and inhibit apoptosis by altering the levels of cleaved caspase-3, cleaved caspase-9, Bax, and Bcl-2 in IDECs exposed to H<sub>2</sub>O<sub>2</sub>.https://www.mdpi.com/1420-3049/27/11/3542resveratrolepithelial cellsduckoxidative stressapoptosis |
| spellingShingle | Ning Zhou Yong Tian Hongzhi Wu Yongqing Cao Ruiqing Li Kang Zou Wenwu Xu Lizhi Lu Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H<sub>2</sub>O<sub>2</sub> Molecules resveratrol epithelial cells duck oxidative stress apoptosis |
| title | Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_full | Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_fullStr | Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_full_unstemmed | Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_short | Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_sort | protective effect of resveratrol on immortalized duck intestinal epithelial cells exposed to h sub 2 sub o sub 2 sub |
| topic | resveratrol epithelial cells duck oxidative stress apoptosis |
| url | https://www.mdpi.com/1420-3049/27/11/3542 |
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