Transcribing Genes the Hard Way: In Vitro Reconstitution of Nanoarchaeal RNA Polymerase Reveals Unusual Active Site Properties

Transcribing Genes the Hard Way: In Vitro Reconstitution of Nanoarchaeal RNA Polymerase Reveals Unusual Active Site Properties

Nanoarchaea represent a highly diverged archaeal phylum that displays many unusual biological features. The Nanoarchaeum equitans genome encodes a complete set of RNA polymerase (RNAP) subunits and basal factors. Several of the standard motifs in the active center contain radical substitutions that...

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Bibliographic Details
Main Authors: Sven Nottebaum, Robert O. J. Weinzierl
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-05-01
Series:Frontiers in Molecular Biosciences
Subjects:
archaea
nanoarchaea
RNA polymerase
catalytic center
active site
high-throughput assay
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2021.669314/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2021.669314/full

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