Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans

Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans

Summary: The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germl...

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Bibliographic Details
Main Authors: Larissa L.S. Scholte, David J. Leggat, Kristen W. Cohen, Lara Hoeweler, Guacyara C. Erwin, Farhard Rahaman, Angela Lombardo, Vincent Philiponis, Dagna S. Laufer, Heather Siefers, Alexis M. Ruppel, Joshua Brand, Janine Maenza, Rhi Bronson, Madhu Prabhakaran, Jalen Jean-Baptiste, Orpheus Kolokythas, Aimee A. Desrosiers, Caroline K. Thoreson, Antje Heit, Nadia J. Khati, Elissa Malkin, M. Juliana McElrath, Adrian B. McDermott, William R. Schief, David Diemert, Jeffrey M. Bethony
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Cell Isolation
Clinical Protocol
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723005439

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http://www.sciencedirect.com/science/article/pii/S2666166723005439

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