Generation of HepG2 Cells with High Expression of Multiple Drug-Metabolizing Enzymes for Drug Discovery Research Using a PITCh System

Generation of HepG2 Cells with High Expression of Multiple Drug-Metabolizing Enzymes for Drug Discovery Research Using a PITCh System

HepG2 cells are an inexpensive hepatocyte model that can be used for repeated experiments, but HepG2 cells do not express major cytochrome P450s (CYPs) and UDP glucuronosyltransferase family 1 member A1 (UGT1A1). In this study, we established CYP3A4–POR–UGT1A1–CYP1A2–CYP2C19–CYP2C9–CYP2D6 (CYPs–UGT1...

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Bibliographic Details
Main Authors: Ryosuke Negoro, Mitsuki Tasaka, Sayaka Deguchi, Kazuo Takayama, Takuya Fujita
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Cells
Subjects:
genome editing
CRISPR-Cas9
PITCh system
HepG2 cell
primary human hepatocytes
CYP1A2
Online Access:https://www.mdpi.com/2073-4409/11/10/1677

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https://www.mdpi.com/2073-4409/11/10/1677

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