Modification of a Constitutive to Glucose-Responsive Liver-Specific Promoter Resulted in Increased Efficacy of Adeno-Associated Virus Serotype 8-Insulin Gene Therapy of Diabetic Mice

Modification of a Constitutive to Glucose-Responsive Liver-Specific Promoter Resulted in Increased Efficacy of Adeno-Associated Virus Serotype 8-Insulin Gene Therapy of Diabetic Mice

We have previously used a hepatotropic adeno-associated viral (AAV) vector with a modified human insulin gene to treat diabetic mice. The HLP (hybrid liver-specific promoter) used was constitutively active and non-responsive to glucose. In this study, we examined the effects of addition of glucose r...

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Bibliographic Details
Main Authors: Kian Chuan Sia, Zhen Ying Fu, Roy Y. Calne, Amit C. Nathwani, Kok Onn Lee, Shu Uin Gan
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Cells
Subjects:
liver-specific glucose-responsive promoter
adeno-associated virus serotype 8 (AAV8)
diabetes gene therapy
long-term basal insulin expression
glucose responsive element (GlRE)
albumin enhancer (3′iALB)
Online Access:https://www.mdpi.com/2073-4409/9/11/2474

Internet

https://www.mdpi.com/2073-4409/9/11/2474

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