Overlap extension cloning of PCR products into a Gateway-compatible plasmid vector
A PCR cloning method that combines a dual selection pGATE-1 plasmid vector and an improved overlap extension cloning was developed. This efficient and cost-effective method allows for the introduction of DNA fragments into the Gateway cloning pipeline. The cloning efficiency is facilitated by a dual...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Future Science Ltd
2023-07-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0001 |