Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible <i>Hsp70Bb</i> promoter in <i>Drosophila melanogaster</i>
AbstractHeat-shock-inducible expression of genes through the use of heat-inducible promoters is commonly used in research despite leaky expression of downstream genes of interest without targeted induction (i.e. heat shock). The development of non-leaky inducible expression systems i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2023-01-01
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| Series: | G3: Genes, Genomes, Genetics |
| Online Access: | https://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkad024 |
| _version_ | 1797788858379665408 |
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| author | Natalie Warsinger-Pepe Carly Chang Connor R Desroberts Omar S Akbari |
| author_facet | Natalie Warsinger-Pepe Carly Chang Connor R Desroberts Omar S Akbari |
| author_sort | Natalie Warsinger-Pepe |
| collection | DOAJ |
| description |
AbstractHeat-shock-inducible expression of genes through the use of heat-inducible promoters is commonly used in research despite leaky expression of downstream genes of interest without targeted induction (i.e. heat shock). The development of non-leaky inducible expression systems is of broad interest for both basic and applied studies, to precisely control gene expression. Here we characterize the use of Polycomb response elements and the inducible Heat-shock protein 70BbDrosophila melanogasterCas9Heat-shock protein 70BbCas9Cas9Cas9 |
| first_indexed | 2024-03-13T01:42:32Z |
| format | Article |
| id | doaj.art-364a8e77c8ef49f186d0567fbd6ee1d9 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-03-13T01:42:32Z |
| publishDate | 2023-01-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-364a8e77c8ef49f186d0567fbd6ee1d92023-07-03T11:13:31ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362023-01-0113410.1093/g3journal/jkad024Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible <i>Hsp70Bb</i> promoter in <i>Drosophila melanogaster</i> Natalie Warsinger-Pepe0Carly Chang1Connor R Desroberts2Omar S Akbari3School of Biological Sciences, Department of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USASchool of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USASchool of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USASchool of Biological Sciences, Department of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA AbstractHeat-shock-inducible expression of genes through the use of heat-inducible promoters is commonly used in research despite leaky expression of downstream genes of interest without targeted induction (i.e. heat shock). The development of non-leaky inducible expression systems is of broad interest for both basic and applied studies, to precisely control gene expression. Here we characterize the use of Polycomb response elements and the inducible Heat-shock protein 70BbDrosophila melanogasterCas9Heat-shock protein 70BbCas9Cas9Cas9https://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkad024 |
| spellingShingle | Natalie Warsinger-Pepe Carly Chang Connor R Desroberts Omar S Akbari Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible <i>Hsp70Bb</i> promoter in <i>Drosophila melanogaster</i> G3: Genes, Genomes, Genetics |
| title | Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible <i>Hsp70Bb</i> promoter in <i>Drosophila melanogaster</i>
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| title_full | Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible <i>Hsp70Bb</i> promoter in <i>Drosophila melanogaster</i>
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| title_fullStr | Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible <i>Hsp70Bb</i> promoter in <i>Drosophila melanogaster</i>
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| title_full_unstemmed | Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible <i>Hsp70Bb</i> promoter in <i>Drosophila melanogaster</i>
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| title_short | Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible <i>Hsp70Bb</i> promoter in <i>Drosophila melanogaster</i>
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| title_sort | polycomb response elements reduce leaky expression of cas9 under temperature inducible i hsp70bb i promoter in i drosophila melanogaster i |
| url | https://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkad024 |
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