Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding

Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding

Abstract Background While pooled loss- and gain-of-function CRISPR screening approaches have become increasingly popular to systematically investigate mammalian gene function, the large majority of them have thus far not investigated the influence of cellular heterogeneity on screen results. Instead...

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Bibliographic Details
Main Authors: Michael Boettcher, Sergio Covarrubias, Anne Biton, James Blau, Haopeng Wang, Noah Zaitlen, Michael T. McManus
Format: Article
Language:English
Published: BMC 2019-02-01
Series:BMC Genomics
Subjects:
CRISPR
Screening
Genome editing
Clonal heterogeneity
Online Access:http://link.springer.com/article/10.1186/s12864-019-5480-0

Internet

http://link.springer.com/article/10.1186/s12864-019-5480-0

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