Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding
Abstract Background While pooled loss- and gain-of-function CRISPR screening approaches have become increasingly popular to systematically investigate mammalian gene function, the large majority of them have thus far not investigated the influence of cellular heterogeneity on screen results. Instead...
Main Authors: | Michael Boettcher, Sergio Covarrubias, Anne Biton, James Blau, Haopeng Wang, Noah Zaitlen, Michael T. McManus |
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Format: | Article |
Language: | English |
Published: |
BMC
2019-02-01
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Series: | BMC Genomics |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12864-019-5480-0 |
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