The inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2 in vitro.
SARS-CoV-2 enters host cells when the viral spike protein is cleaved by transmembrane protease serine 2 (TMPRSS2) after binding to the host angiotensin-converting enzyme 2 (ACE2). Since ACE2 and TMPRSS2 are expressed in the tongue and gingival mucosa, the oral cavity is a potential entry point for S...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0257705 |
| _version_ | 1818719386593132544 |
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| author | Riho Tateyama-Makino Mari Abe-Yutori Taku Iwamoto Kota Tsutsumi Motonori Tsuji Satoru Morishita Kei Kurita Yukio Yamamoto Eiji Nishinaga Keiichi Tsukinoki |
| author_facet | Riho Tateyama-Makino Mari Abe-Yutori Taku Iwamoto Kota Tsutsumi Motonori Tsuji Satoru Morishita Kei Kurita Yukio Yamamoto Eiji Nishinaga Keiichi Tsukinoki |
| author_sort | Riho Tateyama-Makino |
| collection | DOAJ |
| description | SARS-CoV-2 enters host cells when the viral spike protein is cleaved by transmembrane protease serine 2 (TMPRSS2) after binding to the host angiotensin-converting enzyme 2 (ACE2). Since ACE2 and TMPRSS2 are expressed in the tongue and gingival mucosa, the oral cavity is a potential entry point for SARS-CoV-2. This study evaluated the inhibitory effects of general ingredients of toothpastes and mouthwashes on the spike protein-ACE2 interaction and the TMPRSS2 protease activity using an in vitro assay. Both assays detected inhibitory effects of sodium tetradecene sulfonate, sodium N-lauroyl-N-methyltaurate, sodium N-lauroylsarcosinate, sodium dodecyl sulfate, and copper gluconate. Molecular docking simulations suggested that these ingredients could bind to inhibitor-binding site of ACE2. Furthermore, tranexamic acid exerted inhibitory effects on TMPRSS2 protease activity. Our findings suggest that these toothpaste and mouthwash ingredients could help prevent SARS-CoV-2 infection. |
| first_indexed | 2024-12-17T20:06:07Z |
| format | Article |
| id | doaj.art-365d5128d3c246e2b88624dab652c34b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T20:06:07Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-365d5128d3c246e2b88624dab652c34b2022-12-21T21:34:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01169e025770510.1371/journal.pone.0257705The inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2 in vitro.Riho Tateyama-MakinoMari Abe-YutoriTaku IwamotoKota TsutsumiMotonori TsujiSatoru MorishitaKei KuritaYukio YamamotoEiji NishinagaKeiichi TsukinokiSARS-CoV-2 enters host cells when the viral spike protein is cleaved by transmembrane protease serine 2 (TMPRSS2) after binding to the host angiotensin-converting enzyme 2 (ACE2). Since ACE2 and TMPRSS2 are expressed in the tongue and gingival mucosa, the oral cavity is a potential entry point for SARS-CoV-2. This study evaluated the inhibitory effects of general ingredients of toothpastes and mouthwashes on the spike protein-ACE2 interaction and the TMPRSS2 protease activity using an in vitro assay. Both assays detected inhibitory effects of sodium tetradecene sulfonate, sodium N-lauroyl-N-methyltaurate, sodium N-lauroylsarcosinate, sodium dodecyl sulfate, and copper gluconate. Molecular docking simulations suggested that these ingredients could bind to inhibitor-binding site of ACE2. Furthermore, tranexamic acid exerted inhibitory effects on TMPRSS2 protease activity. Our findings suggest that these toothpaste and mouthwash ingredients could help prevent SARS-CoV-2 infection.https://doi.org/10.1371/journal.pone.0257705 |
| spellingShingle | Riho Tateyama-Makino Mari Abe-Yutori Taku Iwamoto Kota Tsutsumi Motonori Tsuji Satoru Morishita Kei Kurita Yukio Yamamoto Eiji Nishinaga Keiichi Tsukinoki The inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2 in vitro. PLoS ONE |
| title | The inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2 in vitro. |
| title_full | The inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2 in vitro. |
| title_fullStr | The inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2 in vitro. |
| title_full_unstemmed | The inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2 in vitro. |
| title_short | The inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2 in vitro. |
| title_sort | inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the sars cov 2 spike protein and ace2 and the protease activity of tmprss2 in vitro |
| url | https://doi.org/10.1371/journal.pone.0257705 |
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