High-resolution description of antibody heavy-chain repertoires in humans.
Antibodies' protective, pathological, and therapeutic properties result from their considerable diversity. This diversity is almost limitless in potential, but actual diversity is still poorly understood. Here we use deep sequencing to characterize the diversity of the heavy-chain CDR3 region,...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3150326?pdf=render |
| _version_ | 1819019677346562048 |
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| author | Ramy Arnaout William Lee Patrick Cahill Tracey Honan Todd Sparrow Michael Weiand Chad Nusbaum Klaus Rajewsky Sergei B Koralov |
| author_facet | Ramy Arnaout William Lee Patrick Cahill Tracey Honan Todd Sparrow Michael Weiand Chad Nusbaum Klaus Rajewsky Sergei B Koralov |
| author_sort | Ramy Arnaout |
| collection | DOAJ |
| description | Antibodies' protective, pathological, and therapeutic properties result from their considerable diversity. This diversity is almost limitless in potential, but actual diversity is still poorly understood. Here we use deep sequencing to characterize the diversity of the heavy-chain CDR3 region, the most important contributor to antibody binding specificity, and the constituent V, D, and J segments that comprise it. We find that, during the stepwise D-J and then V-DJ recombination events, the choice of D and J segments exert some bias on each other; however, we find the choice of the V segment is essentially independent of both. V, D, and J segments are utilized with different frequencies, resulting in a highly skewed representation of VDJ combinations in the repertoire. Nevertheless, the pattern of segment usage was almost identical between two different individuals. The pattern of V, D, and J segment usage and recombination was insufficient to explain overlap that was observed between the two individuals' CDR3 repertoires. Finally, we find that while there are a near-infinite number of heavy-chain CDR3s in principle, there are about 3-9 million in the blood of an adult human being. |
| first_indexed | 2024-12-21T03:39:07Z |
| format | Article |
| id | doaj.art-366191ef8b674e9092ec9288e83a5c4b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T03:39:07Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-366191ef8b674e9092ec9288e83a5c4b2022-12-21T19:17:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2236510.1371/journal.pone.0022365High-resolution description of antibody heavy-chain repertoires in humans.Ramy ArnaoutWilliam LeePatrick CahillTracey HonanTodd SparrowMichael WeiandChad NusbaumKlaus RajewskySergei B KoralovAntibodies' protective, pathological, and therapeutic properties result from their considerable diversity. This diversity is almost limitless in potential, but actual diversity is still poorly understood. Here we use deep sequencing to characterize the diversity of the heavy-chain CDR3 region, the most important contributor to antibody binding specificity, and the constituent V, D, and J segments that comprise it. We find that, during the stepwise D-J and then V-DJ recombination events, the choice of D and J segments exert some bias on each other; however, we find the choice of the V segment is essentially independent of both. V, D, and J segments are utilized with different frequencies, resulting in a highly skewed representation of VDJ combinations in the repertoire. Nevertheless, the pattern of segment usage was almost identical between two different individuals. The pattern of V, D, and J segment usage and recombination was insufficient to explain overlap that was observed between the two individuals' CDR3 repertoires. Finally, we find that while there are a near-infinite number of heavy-chain CDR3s in principle, there are about 3-9 million in the blood of an adult human being.http://europepmc.org/articles/PMC3150326?pdf=render |
| spellingShingle | Ramy Arnaout William Lee Patrick Cahill Tracey Honan Todd Sparrow Michael Weiand Chad Nusbaum Klaus Rajewsky Sergei B Koralov High-resolution description of antibody heavy-chain repertoires in humans. PLoS ONE |
| title | High-resolution description of antibody heavy-chain repertoires in humans. |
| title_full | High-resolution description of antibody heavy-chain repertoires in humans. |
| title_fullStr | High-resolution description of antibody heavy-chain repertoires in humans. |
| title_full_unstemmed | High-resolution description of antibody heavy-chain repertoires in humans. |
| title_short | High-resolution description of antibody heavy-chain repertoires in humans. |
| title_sort | high resolution description of antibody heavy chain repertoires in humans |
| url | http://europepmc.org/articles/PMC3150326?pdf=render |
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