Three-dimensional visualization and analysis of dendritic spines in human brain tissue

We developed a simple yet powerful technique to visualize neuronal morphology in human brain tissues. By ballistically shooting DiI (1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine perchlorate)-coated tungsten particles to randomly label neurons, then clearing tissues with OPTIC...

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Bibliographic Details
Main Authors: Haitao Sun, Hei Ming Lai, Wutian Wu
Format: Article
Language:English
Published: Future Science Ltd 2024-01-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/btn-2023-0078