Three-dimensional visualization and analysis of dendritic spines in human brain tissue
We developed a simple yet powerful technique to visualize neuronal morphology in human brain tissues. By ballistically shooting DiI (1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine perchlorate)-coated tungsten particles to randomly label neurons, then clearing tissues with OPTIC...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Future Science Ltd
2024-01-01
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Series: | BioTechniques |
Subjects: | |
Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0078 |