First study of sperm mediated gene transfer in Egyptian river buffalo

First study of sperm mediated gene transfer in Egyptian river buffalo

The present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmi...

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Bibliographic Details
Main Authors: Mohamed S. Hassanane, Aida I. El Makawy, Sahar M. Helalia, Ahmed S. Abdoon, Kamal M.A. Khalil, Tharwat A. Ghanem, Amany M. Tohamy, Xiao-Feng Sun, Wei Shen
Format: Article
Language:English
Published: Elsevier 2017-12-01
Series:Journal of Genetic Engineering and Biotechnology
Subjects:
Buffalo embryos
Transgenic
SMGT
EGFP
DMSO
Online Access:http://www.sciencedirect.com/science/article/pii/S1687157X17300318

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http://www.sciencedirect.com/science/article/pii/S1687157X17300318

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