First study of sperm mediated gene transfer in Egyptian river buffalo
The present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-12-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X17300318 |
| _version_ | 1797203833872449536 |
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| author | Mohamed S. Hassanane Aida I. El Makawy Sahar M. Helalia Ahmed S. Abdoon Kamal M.A. Khalil Tharwat A. Ghanem Amany M. Tohamy Xiao-Feng Sun Wei Shen |
| author_facet | Mohamed S. Hassanane Aida I. El Makawy Sahar M. Helalia Ahmed S. Abdoon Kamal M.A. Khalil Tharwat A. Ghanem Amany M. Tohamy Xiao-Feng Sun Wei Shen |
| author_sort | Mohamed S. Hassanane |
| collection | DOAJ |
| description | The present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmid DNA concentration, sperm concentration, transfecting agent concentration: Dimethyle sulphoxide (DMSO) and time of transfection. The study proved that the best conditions for producing transgenic embryos were incubation sperm solution its concentration is 107/ml sperm with 3% DMSO: with 20 µg/ml from the linarized DNA, for 15 min at 4 °C are the best conditions to produce transgenic buffalo embryo using sperm mediated gene transfer. |
| first_indexed | 2024-04-24T08:25:37Z |
| format | Article |
| id | doaj.art-367b7c5b8c434d70a3211c9ac64efed2 |
| institution | Directory Open Access Journal |
| issn | 1687-157X |
| language | English |
| last_indexed | 2024-04-24T08:25:37Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-367b7c5b8c434d70a3211c9ac64efed22024-04-16T22:40:02ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2017-12-0115247548210.1016/j.jgeb.2017.06.003First study of sperm mediated gene transfer in Egyptian river buffaloMohamed S. Hassanane0Aida I. El Makawy1Sahar M. Helalia2Ahmed S. Abdoon3Kamal M.A. Khalil4Tharwat A. Ghanem5Amany M. Tohamy6Xiao-Feng Sun7Wei Shen8Cell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, EgyptCell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, EgyptCell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, EgyptAnimal Reproduction and Artificial Insemination Department, Veterinary Research Division, National Research Centre, EgyptGenetics and Cytology Dept., Genetic Engineering and Biotechnology Division, National Research Centre, EgyptZoology Department, Faculty of Science, Helwan University, EgyptZoology Department, Faculty of Science, Helwan University, EgyptInstitute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaInstitute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaThe present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmid DNA concentration, sperm concentration, transfecting agent concentration: Dimethyle sulphoxide (DMSO) and time of transfection. The study proved that the best conditions for producing transgenic embryos were incubation sperm solution its concentration is 107/ml sperm with 3% DMSO: with 20 µg/ml from the linarized DNA, for 15 min at 4 °C are the best conditions to produce transgenic buffalo embryo using sperm mediated gene transfer.http://www.sciencedirect.com/science/article/pii/S1687157X17300318Buffalo embryosTransgenicSMGTEGFPDMSO |
| spellingShingle | Mohamed S. Hassanane Aida I. El Makawy Sahar M. Helalia Ahmed S. Abdoon Kamal M.A. Khalil Tharwat A. Ghanem Amany M. Tohamy Xiao-Feng Sun Wei Shen First study of sperm mediated gene transfer in Egyptian river buffalo Journal of Genetic Engineering and Biotechnology Buffalo embryos Transgenic SMGT EGFP DMSO |
| title | First study of sperm mediated gene transfer in Egyptian river buffalo |
| title_full | First study of sperm mediated gene transfer in Egyptian river buffalo |
| title_fullStr | First study of sperm mediated gene transfer in Egyptian river buffalo |
| title_full_unstemmed | First study of sperm mediated gene transfer in Egyptian river buffalo |
| title_short | First study of sperm mediated gene transfer in Egyptian river buffalo |
| title_sort | first study of sperm mediated gene transfer in egyptian river buffalo |
| topic | Buffalo embryos Transgenic SMGT EGFP DMSO |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X17300318 |
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