Fractionation of acacia honey affects its antioxidant potential in vitro
Objective: To investigate the effects of fractionation of acacia honey on its antioxidant potential in contrast with the pure honey from whole blood, brain and liver in vitro. Methods: Honey was partitioned into three fractions (dichloromethane, ethyl acetate and aqueous). Their immuno-modulatory ef...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2012-01-01
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| Series: | Journal of Acute Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S222161891360027X |
| _version_ | 1818990192597401600 |
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| author | Muhammad Aliyu Oyeronke A. Odunola Ahsana D. Farooq Ahmed M. Mesaik Muhammad I. Choudhary Ochuko L. Erukainure Shahida Perveen Almas Jabeen |
| author_facet | Muhammad Aliyu Oyeronke A. Odunola Ahsana D. Farooq Ahmed M. Mesaik Muhammad I. Choudhary Ochuko L. Erukainure Shahida Perveen Almas Jabeen |
| author_sort | Muhammad Aliyu |
| collection | DOAJ |
| description | Objective: To investigate the effects of fractionation of acacia honey on its antioxidant potential in contrast with the pure honey from whole blood, brain and liver in vitro.
Methods: Honey was partitioned into three fractions (dichloromethane, ethyl acetate and aqueous). Their immuno-modulatory effect on whole blood was assayed using Luminol-amplified chemiluminescence technique. Their antioxidant activities on rat brain and hepatic tissues which covers for catalase, SOD activities and lipid peroxidation.
Results: Fractions of the honey enhanced the production of radicals with no significant (P>0.05) antioxidant activity on whole blood where as pure honey does. Pure honey significantly (P<0.05) stimulates SOD and Catalase activity with no significant (P>0.05) effects on lipid peroxidation.
Conclusions: Fractionation of acacia honey negatively affects its antioxidant potential thereby making it a radical generating agent in contrast with the unfractionated. |
| first_indexed | 2024-12-20T19:50:28Z |
| format | Article |
| id | doaj.art-36816a6900154a31b8f4bb85d92ba72f |
| institution | Directory Open Access Journal |
| issn | 2221-6189 |
| language | English |
| last_indexed | 2024-12-20T19:50:28Z |
| publishDate | 2012-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Acute Disease |
| spelling | doaj.art-36816a6900154a31b8f4bb85d92ba72f2022-12-21T19:28:19ZengWolters Kluwer Medknow PublicationsJournal of Acute Disease2221-61892012-01-011211511910.1016/S2221-6189(13)60027-XFractionation of acacia honey affects its antioxidant potential in vitroMuhammad Aliyu0Oyeronke A. Odunola1Ahsana D. Farooq2Ahmed M. Mesaik3Muhammad I. Choudhary4Ochuko L. Erukainure5Shahida Perveen6Almas Jabeen7Department of Biochemistry, University of Ibadan, Ibadan, Oyo State, NigeriaDepartment of Biochemistry, University of Ibadan, Ibadan, Oyo State, NigeriaH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, PakistanDr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, PakistanH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, PakistanH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, PakistanThird World Centre, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, PakistanDr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, PakistanObjective: To investigate the effects of fractionation of acacia honey on its antioxidant potential in contrast with the pure honey from whole blood, brain and liver in vitro. Methods: Honey was partitioned into three fractions (dichloromethane, ethyl acetate and aqueous). Their immuno-modulatory effect on whole blood was assayed using Luminol-amplified chemiluminescence technique. Their antioxidant activities on rat brain and hepatic tissues which covers for catalase, SOD activities and lipid peroxidation. Results: Fractions of the honey enhanced the production of radicals with no significant (P>0.05) antioxidant activity on whole blood where as pure honey does. Pure honey significantly (P<0.05) stimulates SOD and Catalase activity with no significant (P>0.05) effects on lipid peroxidation. Conclusions: Fractionation of acacia honey negatively affects its antioxidant potential thereby making it a radical generating agent in contrast with the unfractionated.http://www.sciencedirect.com/science/article/pii/S222161891360027XAcacia honeyLipid peroxidationCatalaseSODFractions |
| spellingShingle | Muhammad Aliyu Oyeronke A. Odunola Ahsana D. Farooq Ahmed M. Mesaik Muhammad I. Choudhary Ochuko L. Erukainure Shahida Perveen Almas Jabeen Fractionation of acacia honey affects its antioxidant potential in vitro Journal of Acute Disease Acacia honey Lipid peroxidation Catalase SOD Fractions |
| title | Fractionation of acacia honey affects its antioxidant potential in vitro |
| title_full | Fractionation of acacia honey affects its antioxidant potential in vitro |
| title_fullStr | Fractionation of acacia honey affects its antioxidant potential in vitro |
| title_full_unstemmed | Fractionation of acacia honey affects its antioxidant potential in vitro |
| title_short | Fractionation of acacia honey affects its antioxidant potential in vitro |
| title_sort | fractionation of acacia honey affects its antioxidant potential in vitro |
| topic | Acacia honey Lipid peroxidation Catalase SOD Fractions |
| url | http://www.sciencedirect.com/science/article/pii/S222161891360027X |
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