Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug Absorption
Data on gastric pH in rats to be used in preclinical models for pH-dependent drug absorption are still limited or contradictory. The aim of this study was to describe gastric pH in rats at fasted state and to evaluate its changes induced by pentagastrin or omeprazole in order to mimic gastric pH at...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Karolinum Press
2019-01-01
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| Series: | Prague Medical Report |
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| Online Access: | https://pmr.lf1.cuni.cz/120/1/0005/ |
| _version_ | 1818575607991107584 |
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| author | Martin Šíma Nikolina Kutinová-Canová Pavel Ryšánek Jana Hořínková Denisa Moškořová Ondřej Slanař |
| author_facet | Martin Šíma Nikolina Kutinová-Canová Pavel Ryšánek Jana Hořínková Denisa Moškořová Ondřej Slanař |
| author_sort | Martin Šíma |
| collection | DOAJ |
| description | Data on gastric pH in rats to be used in preclinical models for pH-dependent drug absorption are still limited or contradictory. The aim of this study was to describe gastric pH in rats at fasted state and to evaluate its changes induced by pentagastrin or omeprazole in order to mimic gastric pH at fasted and fed human subjects. Twenty Wistar rats, fasting for 12 h, were randomly assigned into four treatment groups (n=5): control, pre-treated with omeprazole 2 h before pH measurement, pre-treated with omeprazole 12 h before pH measurement, and pre-treated with pentagastrin 20 min before pH measurement. An incision on the stomach wall was made in anesthetized animals, and pH of gastric juice was measured. The observed pH values were significantly different among groups (p=0.0341), with the median (IQR) values of gastric pH of 3.5 (2.7–4.2), 6.7 (4.7–7.0), 5.6 (3.5–6.4) and 2.2 (1.6–3.1) in control, omeprazole 2 h, omeprazole 12 h and pentagastin group, respectively. We recommend using short interval pentagastrin and 2 h omeprazole pre-treatment in fasting animals to model similar gastric pH as is expected in human fasted and fed state pharmacokinetic studies, respectively. |
| first_indexed | 2024-12-15T00:43:15Z |
| format | Article |
| id | doaj.art-36acc845af964e239dfff7493a285d1c |
| institution | Directory Open Access Journal |
| issn | 1214-6994 2336-2936 |
| language | English |
| last_indexed | 2024-12-15T00:43:15Z |
| publishDate | 2019-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Prague Medical Report |
| spelling | doaj.art-36acc845af964e239dfff7493a285d1c2022-12-21T22:41:36ZengKarolinum PressPrague Medical Report1214-69942336-29362019-01-0112015910.14712/23362936.2019.5Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug AbsorptionMartin Šíma0Nikolina Kutinová-Canová1Pavel Ryšánek2Jana Hořínková3Denisa Moškořová4Ondřej Slanař5Institute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech RepublicInstitute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech RepublicInstitute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech RepublicInstitute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech RepublicInstitute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech RepublicInstitute of Pharmacology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech RepublicData on gastric pH in rats to be used in preclinical models for pH-dependent drug absorption are still limited or contradictory. The aim of this study was to describe gastric pH in rats at fasted state and to evaluate its changes induced by pentagastrin or omeprazole in order to mimic gastric pH at fasted and fed human subjects. Twenty Wistar rats, fasting for 12 h, were randomly assigned into four treatment groups (n=5): control, pre-treated with omeprazole 2 h before pH measurement, pre-treated with omeprazole 12 h before pH measurement, and pre-treated with pentagastrin 20 min before pH measurement. An incision on the stomach wall was made in anesthetized animals, and pH of gastric juice was measured. The observed pH values were significantly different among groups (p=0.0341), with the median (IQR) values of gastric pH of 3.5 (2.7–4.2), 6.7 (4.7–7.0), 5.6 (3.5–6.4) and 2.2 (1.6–3.1) in control, omeprazole 2 h, omeprazole 12 h and pentagastin group, respectively. We recommend using short interval pentagastrin and 2 h omeprazole pre-treatment in fasting animals to model similar gastric pH as is expected in human fasted and fed state pharmacokinetic studies, respectively.https://pmr.lf1.cuni.cz/120/1/0005/Gastric pHRatsOral drug absorptionOmeprazolePentagastrin |
| spellingShingle | Martin Šíma Nikolina Kutinová-Canová Pavel Ryšánek Jana Hořínková Denisa Moškořová Ondřej Slanař Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug Absorption Prague Medical Report Gastric pH Rats Oral drug absorption Omeprazole Pentagastrin |
| title | Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug Absorption |
| title_full | Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug Absorption |
| title_fullStr | Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug Absorption |
| title_full_unstemmed | Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug Absorption |
| title_short | Gastric pH in Rats: Key Determinant for Preclinical Evaluation of pH-dependent Oral Drug Absorption |
| title_sort | gastric ph in rats key determinant for preclinical evaluation of ph dependent oral drug absorption |
| topic | Gastric pH Rats Oral drug absorption Omeprazole Pentagastrin |
| url | https://pmr.lf1.cuni.cz/120/1/0005/ |
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