L-lactate exerts a pro-proliferative effect on adult hippocampal precursor cells in vitro
Summary: L-lactate has energetic and signaling properties, and its availability is modulated by activity-dependent stimuli, which also regulate adult hippocampal neurogenesis. Studying the effects of L-lactate on neural precursor cells (NPCs) in vitro, we found that L-lactate is pro-proliferative an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-02-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221000948 |
| _version_ | 1818877875675201536 |
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| author | Alexandra Pötzsch Sara Zocher Stefanie N. Bernas Odette Leiter Annette E. Rünker Gerd Kempermann |
| author_facet | Alexandra Pötzsch Sara Zocher Stefanie N. Bernas Odette Leiter Annette E. Rünker Gerd Kempermann |
| author_sort | Alexandra Pötzsch |
| collection | DOAJ |
| description | Summary: L-lactate has energetic and signaling properties, and its availability is modulated by activity-dependent stimuli, which also regulate adult hippocampal neurogenesis. Studying the effects of L-lactate on neural precursor cells (NPCs) in vitro, we found that L-lactate is pro-proliferative and that this effect is dependent on the active lactate transport by monocarboxylate transporters. Increased proliferation was not linked to amplified mitochondrial respiration. Instead, L-lactate deviated glucose metabolism to the pentose phosphate pathway, indicated by increased glucose-6-phosphate dehydrogenase activity while glycolysis decreased. Knockout of Hcar1 revealed that the pro-proliferative effect of L-lactate was not dependent on receptor activity although phosphorylation of ERK1/2 and Akt was increased following L-lactate treatment. Together, we show that availability of L-lactate is linked to the proliferative potential of NPCs and add evidence to the hypothesis that lactate influences cellular homeostatic processes in the adult brain, specifically in the context of adult hippocampal neurogenesis. |
| first_indexed | 2024-12-19T14:05:14Z |
| format | Article |
| id | doaj.art-36cdd62893eb4081aa6a3c4cac2cb190 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-19T14:05:14Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-36cdd62893eb4081aa6a3c4cac2cb1902022-12-21T20:18:19ZengElsevieriScience2589-00422021-02-01242102126L-lactate exerts a pro-proliferative effect on adult hippocampal precursor cells in vitroAlexandra Pötzsch0Sara Zocher1Stefanie N. Bernas2Odette Leiter3Annette E. Rünker4Gerd Kempermann5German Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden, Germany; CRTD – Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden, GermanyGerman Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden, Germany; CRTD – Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden, GermanyGerman Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden, Germany; CRTD – Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden, GermanyCRTD – Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden, GermanyGerman Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden, Germany; CRTD – Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden, GermanyGerman Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden, Germany; CRTD – Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden, Germany; Corresponding authorSummary: L-lactate has energetic and signaling properties, and its availability is modulated by activity-dependent stimuli, which also regulate adult hippocampal neurogenesis. Studying the effects of L-lactate on neural precursor cells (NPCs) in vitro, we found that L-lactate is pro-proliferative and that this effect is dependent on the active lactate transport by monocarboxylate transporters. Increased proliferation was not linked to amplified mitochondrial respiration. Instead, L-lactate deviated glucose metabolism to the pentose phosphate pathway, indicated by increased glucose-6-phosphate dehydrogenase activity while glycolysis decreased. Knockout of Hcar1 revealed that the pro-proliferative effect of L-lactate was not dependent on receptor activity although phosphorylation of ERK1/2 and Akt was increased following L-lactate treatment. Together, we show that availability of L-lactate is linked to the proliferative potential of NPCs and add evidence to the hypothesis that lactate influences cellular homeostatic processes in the adult brain, specifically in the context of adult hippocampal neurogenesis.http://www.sciencedirect.com/science/article/pii/S2589004221000948Biological SciencesHuman MetabolismCell Biology |
| spellingShingle | Alexandra Pötzsch Sara Zocher Stefanie N. Bernas Odette Leiter Annette E. Rünker Gerd Kempermann L-lactate exerts a pro-proliferative effect on adult hippocampal precursor cells in vitro iScience Biological Sciences Human Metabolism Cell Biology |
| title | L-lactate exerts a pro-proliferative effect on adult hippocampal precursor cells in vitro |
| title_full | L-lactate exerts a pro-proliferative effect on adult hippocampal precursor cells in vitro |
| title_fullStr | L-lactate exerts a pro-proliferative effect on adult hippocampal precursor cells in vitro |
| title_full_unstemmed | L-lactate exerts a pro-proliferative effect on adult hippocampal precursor cells in vitro |
| title_short | L-lactate exerts a pro-proliferative effect on adult hippocampal precursor cells in vitro |
| title_sort | l lactate exerts a pro proliferative effect on adult hippocampal precursor cells in vitro |
| topic | Biological Sciences Human Metabolism Cell Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221000948 |
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