A novel salt- and organic solvent-tolerant phosphite dehydrogenase from Cyanothece sp. ATCC 51142

A novel salt- and organic solvent-tolerant phosphite dehydrogenase from Cyanothece sp. ATCC 51142

Phosphite dehydrogenase (PtxD) is a promising enzyme for NAD(P)H regeneration. To expand the usability of PtxD, we cloned, expressed, and analyzed PtxD from the marine cyanobacterium Cyanothece sp. ATCC 51142 (Ct-PtxD). Ct-PtxD exhibited maximum activity at pH 9.0°C and 50°C and high stability over...

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Bibliographic Details
Main Authors: Gamal Nasser Abdel-Hady, Takahisa Tajima, Takeshi Ikeda, Takenori Ishida, Hisakage Funabashi, Akio Kuroda, Ryuichi Hirota
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-08-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
phosphite dehydrogenase
NAD(P)H regeneration
salt resistance
organic solvent tolerance
low water activity
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1255582/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2023.1255582/full

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