Invasive Pathobionts Contribute to Colon Cancer Initiation by Counterbalancing Epithelial Antimicrobial ResponsesSummary
Background & Aims: Microbiota dysbiosis and mucosa-associated bacteria are involved in colorectal cancer progression. We hypothesize that an interaction between virulent pathobionts and epithelial defense promotes tumorigenesis. Methods: Chemical-induced CRC mouse model was treated with anti...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Cellular and Molecular Gastroenterology and Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352345X21001740 |
| _version_ | 1819101941720940544 |
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| author | Linda Chia-Hui Yu Shu-Chen Wei Yi-Hsuan Li Po-Yu Lin Xin-Yu Chang Jui-Ping Weng Yin-Wen Shue Liang-Chuan Lai Jin-Town Wang Yung-Ming Jeng Yen-Hsuan Ni |
| author_facet | Linda Chia-Hui Yu Shu-Chen Wei Yi-Hsuan Li Po-Yu Lin Xin-Yu Chang Jui-Ping Weng Yin-Wen Shue Liang-Chuan Lai Jin-Town Wang Yung-Ming Jeng Yen-Hsuan Ni |
| author_sort | Linda Chia-Hui Yu |
| collection | DOAJ |
| description | Background & Aims: Microbiota dysbiosis and mucosa-associated bacteria are involved in colorectal cancer progression. We hypothesize that an interaction between virulent pathobionts and epithelial defense promotes tumorigenesis. Methods: Chemical-induced CRC mouse model was treated with antibiotics at various phases. Colonic tissues and fecal samples were collected in a time-serial mode and analyzed by gene microarray and 16S rRNA sequencing. Intraepithelial bacteria were isolated using a gentamicin resistance assay, and challenged in epithelial cultures. Results: Our study showed that antibiotic treatment at midphase but not early or late phase reduced mouse tumor burden, suggesting a time-specific host–microbe interplay. A unique antimicrobial transcriptome profile showing an inverse relationship between autophagy and oxidative stress genes was correlated with a transient surge in microbial diversity and virulence emergence in mouse stool during cancer initiation. Gavage with fimA/fimH/htrA-expressing invasive Escherichia coli isolated from colonocytes increased tumor burden in recipient mice, whereas inoculation of bacteria deleted of htrA or triple genes did not. The invasive E. coli suppressed epithelial autophagy activity through reduction of microtubule-associated protein 1 light-chain 3 transcripts and caused dual oxidase 2–dependent free radical overproduction and tumor cell hyperproliferation. A novel alternating spheroid culture model was developed for sequential bacterial challenge to address the long-term changes in host–microbe interaction for chronic tumor growth. Epithelial cells with single bacterial encounter showed a reduction in transcript levels of autophagy genes while those sequentially challenged with invasive E. coli showed heightened autophagy gene expression to eliminate intracellular microbes, implicating that bacteria-dependent cell hyperproliferation could be terminated at late phases. Finally, the presence of bacterial htrA and altered antimicrobial gene expression were observed in human colorectal cancer specimens. Conclusions: Invasive pathobionts contribute to cancer initiation during a key time frame by counterbalancing autophagy and oxidative stress in the colonic epithelium. Monitoring gut microbiota and antimicrobial patterns may help identify the window of opportunity for intervention with bacterium-targeted precision medicine. |
| first_indexed | 2024-12-22T01:26:40Z |
| format | Article |
| id | doaj.art-36e9e921bad64876b037e38f236fee69 |
| institution | Directory Open Access Journal |
| issn | 2352-345X |
| language | English |
| last_indexed | 2024-12-22T01:26:40Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cellular and Molecular Gastroenterology and Hepatology |
| spelling | doaj.art-36e9e921bad64876b037e38f236fee692022-12-21T18:43:35ZengElsevierCellular and Molecular Gastroenterology and Hepatology2352-345X2022-01-011315779Invasive Pathobionts Contribute to Colon Cancer Initiation by Counterbalancing Epithelial Antimicrobial ResponsesSummaryLinda Chia-Hui Yu0Shu-Chen Wei1Yi-Hsuan Li2Po-Yu Lin3Xin-Yu Chang4Jui-Ping Weng5Yin-Wen Shue6Liang-Chuan Lai7Jin-Town Wang8Yung-Ming Jeng9Yen-Hsuan Ni10Graduate Institute of Physiology, Taipei, Taiwan, Republic of ChinaDepartment of Internal Medicine, Taipei, Taiwan, Republic of ChinaGraduate Institute of Physiology, Taipei, Taiwan, Republic of ChinaGraduate Institute of Physiology, Taipei, Taiwan, Republic of ChinaGraduate Institute of Physiology, Taipei, Taiwan, Republic of ChinaGraduate Institute of Physiology, Taipei, Taiwan, Republic of ChinaGraduate Institute of Physiology, Taipei, Taiwan, Republic of China; Department of Internal Medicine, Taipei, Taiwan, Republic of ChinaGraduate Institute of Physiology, Taipei, Taiwan, Republic of China; Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan, Republic of ChinaDepartment of Internal Medicine, Taipei, Taiwan, Republic of China; Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan, Republic of ChinaDepartment of Pathology, Taipei, Taiwan, Republic of ChinaDepartment of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan, Republic of China; Correspondence Address correspondence to: Yen-Hsuan Ni, MD, PhD, Department of Pediatrics, National Taiwan University College of Medicine and Hospital, 7 Chung-Shan South Road, Taipei, Taiwan, Republic of China. fax: (886) 2-23938871.Background & Aims: Microbiota dysbiosis and mucosa-associated bacteria are involved in colorectal cancer progression. We hypothesize that an interaction between virulent pathobionts and epithelial defense promotes tumorigenesis. Methods: Chemical-induced CRC mouse model was treated with antibiotics at various phases. Colonic tissues and fecal samples were collected in a time-serial mode and analyzed by gene microarray and 16S rRNA sequencing. Intraepithelial bacteria were isolated using a gentamicin resistance assay, and challenged in epithelial cultures. Results: Our study showed that antibiotic treatment at midphase but not early or late phase reduced mouse tumor burden, suggesting a time-specific host–microbe interplay. A unique antimicrobial transcriptome profile showing an inverse relationship between autophagy and oxidative stress genes was correlated with a transient surge in microbial diversity and virulence emergence in mouse stool during cancer initiation. Gavage with fimA/fimH/htrA-expressing invasive Escherichia coli isolated from colonocytes increased tumor burden in recipient mice, whereas inoculation of bacteria deleted of htrA or triple genes did not. The invasive E. coli suppressed epithelial autophagy activity through reduction of microtubule-associated protein 1 light-chain 3 transcripts and caused dual oxidase 2–dependent free radical overproduction and tumor cell hyperproliferation. A novel alternating spheroid culture model was developed for sequential bacterial challenge to address the long-term changes in host–microbe interaction for chronic tumor growth. Epithelial cells with single bacterial encounter showed a reduction in transcript levels of autophagy genes while those sequentially challenged with invasive E. coli showed heightened autophagy gene expression to eliminate intracellular microbes, implicating that bacteria-dependent cell hyperproliferation could be terminated at late phases. Finally, the presence of bacterial htrA and altered antimicrobial gene expression were observed in human colorectal cancer specimens. Conclusions: Invasive pathobionts contribute to cancer initiation during a key time frame by counterbalancing autophagy and oxidative stress in the colonic epithelium. Monitoring gut microbiota and antimicrobial patterns may help identify the window of opportunity for intervention with bacterium-targeted precision medicine.http://www.sciencedirect.com/science/article/pii/S2352345X21001740Colon CarcinomaGut MicrobiomeIntracellular MicrobesXenophagyReactive Oxygen SpeciesOrganoids and Spheroids |
| spellingShingle | Linda Chia-Hui Yu Shu-Chen Wei Yi-Hsuan Li Po-Yu Lin Xin-Yu Chang Jui-Ping Weng Yin-Wen Shue Liang-Chuan Lai Jin-Town Wang Yung-Ming Jeng Yen-Hsuan Ni Invasive Pathobionts Contribute to Colon Cancer Initiation by Counterbalancing Epithelial Antimicrobial ResponsesSummary Cellular and Molecular Gastroenterology and Hepatology Colon Carcinoma Gut Microbiome Intracellular Microbes Xenophagy Reactive Oxygen Species Organoids and Spheroids |
| title | Invasive Pathobionts Contribute to Colon Cancer Initiation by Counterbalancing Epithelial Antimicrobial ResponsesSummary |
| title_full | Invasive Pathobionts Contribute to Colon Cancer Initiation by Counterbalancing Epithelial Antimicrobial ResponsesSummary |
| title_fullStr | Invasive Pathobionts Contribute to Colon Cancer Initiation by Counterbalancing Epithelial Antimicrobial ResponsesSummary |
| title_full_unstemmed | Invasive Pathobionts Contribute to Colon Cancer Initiation by Counterbalancing Epithelial Antimicrobial ResponsesSummary |
| title_short | Invasive Pathobionts Contribute to Colon Cancer Initiation by Counterbalancing Epithelial Antimicrobial ResponsesSummary |
| title_sort | invasive pathobionts contribute to colon cancer initiation by counterbalancing epithelial antimicrobial responsessummary |
| topic | Colon Carcinoma Gut Microbiome Intracellular Microbes Xenophagy Reactive Oxygen Species Organoids and Spheroids |
| url | http://www.sciencedirect.com/science/article/pii/S2352345X21001740 |
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