Pretreatment Method for DNA Barcoding to Analyze Gut Contents of Rotifers

We designed an experiment to analyze the gut content of Rotifera based on DNA barcoding and tested it on <i>Asplanchna</i> sp. in order to ensure that the DNA extracted from the rotifer species is from the food sources within the gut. We selected ethanol fixation (60%) to minimize the in...

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Bibliographic Details
Main Authors: Hye-Ji Oh, Paul Henning Krogh, Hyun-Gi Jeong, Gea-Jae Joo, Ihn-Sil Kwak, Sun-Jin Hwang, Jeong-Soo Gim, Kwang-Hyeon Chang, Hyunbin Jo
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/10/3/1064